消乳增胶囊致畸作用研究.PDFVIP

消乳增胶囊致畸作用研究  * 蒲君峰，贾海，张志宏，王辉，葛斌，高湘 (甘肃省人民医院，兰州 730000) 摘要：目的 观察消乳增对大鼠是否存在胚胎毒性和致畸毒性。方法 采用 Wistar 大鼠，每组妊娠鼠15 只。试验设消 乳增高、中、低 3 个剂量组，分别给药 9.13 ，4.55 ，2.28 g·kg1 ，同时设阳性对照组( 阿司匹林组)和空白组。在大鼠胚胎 器官形成期连续灌胃给药 10 d(孕 7~16 d) 。在妊娠的第 20 天，处死妊娠鼠，计数黄体数、胚胎的着床数、活胎数、早期 死胎数( 包括吸收胎)和晚期死胎数。观察胎仔外观、发育情况并称重后，将每窝 1/2 的活胎仔作骨路检查。另取 l/2 活胎 仔固定于 Bouin 氏液进行内脏检查。结果 消乳增对孕鼠体质量影响差异无统计学意义，各给药组与空白组比较，活胎 数显著减少，吸收胎数显著增加，并显著抑制胎鼠体长；并导致胎鼠胸骨骨化不全等骨骼畸形。结论 消乳增对大鼠有 较为明显的胚胎毒性，包括胚胎死亡、生长发育抑制和骨骼畸形等。 关键词：消乳增胶囊；致畸；胚胎毒性；大鼠 中图分类号：R965.3 文献标志码：A 文章编号：1007-7693(2015)04-0404-04 DOI: 10.13748/ki.issn1007-7693.2015.04.004 Study on Teratogenetic Effects of Xiaoruzeng Capsule in Rats * PU Junfeng, JIA Hai, ZHANG Zhihong, WANG Hui, Ge Bin, Gao Xiang (Gansu Provincial Hospital, Lanzhou 730000, China) ABSTRACT: OBJECTIVE To observe the embryotoxicity and teratogenicity of Xiaoruzeng capsule. METHODS Pregnant Wistar rats were randomly divided into five groups and each group had over 15 rats. Three dose groups rats(9.13, 4.55, 2.28 g·kg1) received intragastric administration during 7~16 d of gestation. All the pregnant rats were sacrificed on the pregnancy 20th day and measured number of corpus luteum, implantation, live embryo number, dead embryo and absorption embryo. To measure developmental condition and half of live embryo rats of each nest were dyed by the sodium alizarin sulfonate to inspect the whole body skeleton growth situation, and half of live embryo rats were given the internal organs inspection after the Bouin fluid immersion. RESULTS There w