益肾Ⅰ方抑制大鼠佐剂性关节炎作用及作用机制研讨.pdfVIP

益肾 方抑制大鼠佐剂性关节炎作用及作用机制研究* 1 1 1 1 1 2 2 谭海荣 潘竞锵 肖柳英 郭凤莲 韩超 汤永洪 邱志健 1 广州市中医中药研究所 510130 2 广东药学院药学系 [摘要] 目的 探讨益肾 方治疗佐剂性关节炎的药理作用及其作用机制 方 法 制备佐剂性关节炎大鼠模型 观察益肾 方对病鼠超敏反应性炎症左 右后足 跖厚度以及病鼠血清肿瘤坏死因子 TNF -á 循环免疫复合物 CIC ,丙二醛 MDA 含量和超氧化物歧化酶 SOD 活性的影响 结果 益肾 方能抑制佐剂性关节炎 大鼠继发性 自身免疫性足跖肿胀 疗效随疗程延长而效果愈为明显 差异有高度 显著性 P0.01 降低病鼠增高的TNF-á CIC, MDA 含量 提高其低下的SOD 活 性 差异均有高度显著性 P0.01 结论 益肾 方具有对抗大鼠佐剂性 继发性 关节炎的作用 其机制与抑制自由基损伤和降低自身免疫的病理生理性反应有关 [关键词] 益肾 方 大鼠 佐剂性关节炎 肿瘤坏死因子-á 自由基损伤 Anti-inflammatory mechanism of Yishen formula on adjuvant arthritis in rats 1 1 1 1 1 2 2 TAN Hairong, PAN Jingqiang, XIAO Liuying, GUO Fenglian, HAN Chao, TANG Yonghong QIU Zhijian. 1 Guangzhou Institute of Traditional Chinese Medicine and Materia Medica, Guangzhou, China 510130 2 Guangdong pharmaceutical college, Guangzhou, China 510240 . [Abstract] Objective To investigate the pharmacological effect and mechanism of Yishen formula on rats with adjuvant arthritis (AA). Methods To observe the change of secondary inflammation (secondary inflammatory swelling, multiple arthritis), and level of tumor necrosis factor-á (TNF-á), circulating immune complexes (CIC), malondialdehyde (MDA), antioxidizing activity of superoxide dismutase (SOD) in AA rats treated with the formula. Results In different period of treatment with the formula the swelling paws of secondary immunoreactive inflammation were very significantly inhibited (P0.01), contents of TNF-á, CIC, MDA obviously decreased and the activity of SOD potentiated (P0.01) in AA rats. Conclusion Yishen formula shows visibly anti-infl