真菌（念珠菌kefyr+IFO+0586）细胞壁复杂寡糖的合成研究.pdfVIP

目录和摘要 中文摘要 本论文主要包括两部分内容; 第一部分:以3,4,60一苯甲酞基-a-D一甘露毗喃糖烯丙基普 (5),6-0一乙酞基一3,4一二一O一苯甲酸基一a-D一甘露毗喃糖烯丙基营 (7) 和3,4一二一O一苯甲酞基-a-D一甘露毗喃糖烯丙基普 (9)为合成子，高效 地合成了梳状的酵母念珠菌kefyr-IFO0586的细胞壁甘露多糖重复结 构单元五糖及其二聚体，即a-D一甘露毗喃糖基一((1--2)-a-D一甘露毗喃 糖基一((1-6)-[a一 甘露毗喃糖基一((1-+2)-a-D一甘露毗喃糖基 一(1-+2汗a-D一甘露毗喃糖烯丙基普 (1)和a-D一甘露毗喃糖基 一((1-2)-a-D一甘露毗喃糖基一((1-6)-[a-D一甘露毗喃糖基一((1-2)-a-D一甘 露毗喃糖基一(1-2)]-a。一甘露毗喃糖基一((1--6)一a【-D一甘露毗喃糖基 一((1-2)]-a-D一甘露毗喃糖基一(1--,.-6)-[a-D一甘露毗喃糖基一((1-,.2)-a-D一甘 露毗喃糖基一((I-+2)]-a一甘露毗喃搪烯丙基昔 (2)0 第二部分:发展了一种新的表面附聚薄壳型阳离子色谱柱填料的 简易制备方法。首次报告合成了单分散高效阳离子色谱柱填料。以 lONm的单分散聚苯乙烯一二乙烯基苯 (PSTDVB)微球为基球，对 其表面季胺化，然后均匀附聚一层磺化的阳离子胶乳 ((0.2gm)，得到 阳离子色谱固定相。分别用碱金属(Li+,Na十，NH4十，K+)及碱土金属离 子(Mg2+，Ca2+)对用该填料制成的色谱柱的色谱性能进行了测试，结果 表明该柱填料对常见阳离子具有很好的分离效果，良好的重现性和低 的检测限。 关键词:寡糖;区域和立体选择性合成:阳离子色谱;固定相; 单分散聚合物微球 目录和摘要 ABSTRACT Thisdissertationincludesthefollowingtwoparts. 1. a-D-Mannopyranosyl-(1--2)-a-D-mannopyranosyl-(1-6)一[a-D- mannopyranosyl-(1-2)-a-D-mannopyranosyl-(1--2)]-a-D-mannopyran ose (1)and a-D-mannopyranosyl-(1-2)-a-D-mannopyranosyl 一(1-6)-[a-D-mannopyranosyl-(1--2)-(x-Danannopyranosyl-(I-2)]-a- D-mannopyranosyl-(1-6)一[a-D-mannopyranosyl-(1-2)]-a-D-mannopy ranosyl-(1-}6)一a〔-D-mannopyranosyl-(1-2)-a-D-mannopyranosyl-{1-- 2)]-a-D-mannopyranose(2),therepeatingunitanditsdimmerofthecell wallmannanofthepathogenicyeastCandidakefyrIFO0586,hasbeen efficientlysynthesizedfirstlyastheirallylglycosidesbyusingallyl 3,4,6-tri-O-benzoyl-a-D-mannopyranoside(5),allyl6-O-acetyl-3,4-di-O -benzoyl-a-D-mannopyranoside(7),andallyl3,4-di-O-benzoyl-a-D- mannopyranoside(9)assynthons. 2. Anewandsimplemethodforpreparationoflatex-coatedpellicular cationexchangerwasintroduced.Chromatographicstationaryphaseof monodisperseagglomeratedpellicularcationwaspreparedforthefirst time.Themonodispersepolyvinylbenzene-divinylbenzene(PSTDVB