仙鹤草水提液对人癌细胞的生长抑制作用研讨.pdfVIP

仙鹤草水提液对人癌细胞的生长抑制作用研究 1 2 2 2 2 刘兰茹 吴琳华 郭劲柏 刘红梅 刘伟 1.哈尔滨医科大学药学院 2.哈尔滨医科大学附属第二医院 哈尔滨 150086 摘要 目的 研究仙鹤草水提液对人癌细胞的生长抑制作用 方法 本实验应用 流式细胞技术和电镜技术体外分别研究了仙鹤草水提液对胃癌 BGC-803 和宫颈癌 Hela 细胞株的生长抑制作用和细胞周期的影响 结果 实验结果表明 仙鹤草水 提液在药物浓度为 100µg/ml 时 对胃癌 BGC-803 和宫颈癌 Hela 的抑制率分别为 61.9% 67.9% 流式细胞结果显示仙鹤草水提液可干扰肿瘤细胞周期进程 阻滞 肿瘤细胞由 G1+G0 期向 S 期和 G2+M 期转化 属于细胞周期特异性抗肿瘤药物 结论 实验结果说明仙鹤草作为一种很有前景的抗肿瘤药物 我们应对其做深入 的研究 关键词 仙鹤草水提液 细胞周期 电镜 Inhibition effects of agrimonia water extration on human tumor cell line growth 1 2 1 2 WU Lin-hua , GOU Jin-bai , LIU Hong-mei , LIU Wei (1 Base of clinical Pharmacology of the Second Hospital Affiliated Harbin Medical University, Harbin 150086; Lab of Molecular Biology of the Second Hospital Affiliated Harbin Medical University, Harbin 150086) ABSTRACT: OBJECTIVE To investigate the inhibitory effect of agrimonia water extraction (AWE) on Human tumor cell line growth. METHODS The effects of AWE on human gastric cancer BGC-803 and hela cell line were studied by using electronic microscopy and flow cytometry respectively. RESULTS The results showed that the inhibition rate of gastric cancer BGC-803 and Hela cells were 61.9% and 67.9% in 100 µg.ml-1 of AWE respectively . The results of flow cytometry showed that AWE could interfere the cell cycle and inhibit the transforming of cells from G +G phase to S and 1 0 G2+M phase, called as cell cycle special anti-tumor drugs. CONCLUSION