基于Erns和E2基因的猪瘟标记疫苗研究概述.PDFVIP

微生物学通报 Dec. 20, 2017, 44(12): 3038−3044 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.170513 专论与综述 基于 Erns 和 E2 基因的猪瘟标记疫苗研究概述 刘元杰 邹兴启 徐璐 朱元源 张乾义 王琴 赵启祖* ( 100081) 摘 要：猪瘟(Classical swine fever ，CSF)是猪的一种急性、热性和致死性传染病。该病流行范 围很广，而且致死率极高，给世界养猪业造成严重危害。目前，猪瘟流行地区或国家仍然采用 接种弱毒疫苗的方法作为预防猪瘟的主要策略，但接种弱毒疫苗的传统预防控制方法无法区别 猪瘟疫苗免疫抗体和野毒感染抗体。为了净化、消灭猪瘟，新型标记疫苗的研究已迫在眉睫。 近些年，陆续有国内外研究者应用分子生物学和基因工程方法，对猪瘟野毒株或弱毒株进行基 因修饰构建出新毒株，其中以Erns 和E2 为基础构建新毒株的方法占据着重要地位。部分候选 疫苗具有较好的免疫效果，可用于区分免疫和自然感染动物，而且有望作为新一代疫苗来替代 传统弱毒疫苗。 关键词：猪瘟，标记疫苗，Erns 和E2 ，基因突变，基因重组 Overview of researches on CSF marker vaccine based on Erns and E2 LIU Yuan-Jie ZOU Xing-Qi XU Lu ZHU Yuan-Yuan ZHANG Qian-Yi * WANG Qin ZHAO Qi-Zu (China Institute of Veterinary Drugs Control, Beijing 100081, China) Abstract: Classical swine fever (CSF) is a kind of infectious disease which could induce acute, febrile and fatal disease in swine. This disease displays with wild prevalence and high mortality, and could lead to significant damage for the pig industry all over the world. At present, most CSF epidemic countries or areas still prevent the CSF virus (CSFV) infection mainly by the vaccination of CSFV live-attenuated vaccine (LAV). However, the traditional LAV could not differentiate the CSFV antibody of vaccinated pigs from infected ones, so it is very urgent to develop the new tag vaccines to purge and even eliminate