嗜酸硫杆菌属物种的比较基因组学分析.pdfVIP

42 生 物 技 术 2014年24(6) 氧化亚铁嗜酸硫杆菌还可以通过固氮酶(AFE1522—1515和Lferr一1240—1233)固定氮气获得氮源。此外，插入序列造成硫氧化 还原酶基因的缺失以及7个功能基因失活等现象表明冶金微生物基因组存在不稳定性。[结论]比较基因组学为更好地挖掘嗜 酸硫杆菌属物种的基因组数据信息、揭示其基因组结构特征以及冶金性状等差异提供了重要支持。 关键词：喜温嗜酸硫杆菌，氧化亚铁嗜酸硫杆菌，比较基因组学，生物冶金，硫氧化还原酶 中图分类号：Q71 文献标识码 ：A doi：10．3969／j．issn．1004—311X．2014．06．0135—7 ComparativegenomeanalysisofAcidithiobacillusstrains JingjingLi，MinJiu，ChenyingJiang，LunjiWang，DuchuangWang，XiaoyanYou 1．CollegeofFoodandBioengineering，HenanUniversityofScienceand Technology，Luoyang471023，China；2．StateKeyLaboratoryofMicrobialResources，In— stituteofMicrobiology，ChineseAcademyofSceinces，Being100101，China Abstract：[objective]Comparativegenomicmethodswereutilizedtoanalyzeanddate—miningthegenomefeaturesofAcidithiobaciUus caldusandAcidithiobacillusferrooxida瑚．[Methods]GenomcandwholeproteomesequencesoffourAcidithiobacillusstrainswerealign— mentandvisualizedbyusingtheBlastprogram ，MUMmersoftwarenadArtemisComparisonToo1．Thedeletionofsulfur oxygenasereduc— tase(SOR)genewasvalidatedbytheRT—PCRexperiments．[Results]TherearelargedifferencesbetweenAt．caldus and At．ferrooxidans．Noflagellacoding—genesandleasttransposableelements(26nad41)wereidentifiedintheAt．ferrooxidansge— nomes．Sulfuroxidationsystems(SOX，clusterInadclusterII)wereusedtoRISCs(Reducedinorganicsulfur compounds)oxidationme- tabolisminAt．caldus．Ni~ogenCna befixedbyAt．ferrooxidans(AFE1522—1515andLferr_1240—1233)toprovidenecessaryni~ogen sourceexceptnitratereductionandassimilation．Inaddition，thedeletionofSOR geneandinactivationofsevenfunctional genescausedby theinsertsequencessuggestedtheinstabilityofbioleaehingmicrobialgenome．[Conclusion]Comparativegenomicsprovidesapowerful methodofrgenomedateminingofAcidithiobacillus，whichwillhelptOreveal thedifferencesofgenomestructureandgeneticdifferencesof metbaolism properties． Keywords：Acidithiobacilluscaldus，Acidithiobacillusferrooxidans，compraativegenomies，bioleaching，SOR 0 引言