试验室检测及数据分析应用.ppt

Interpretation of Serological results血清结果的分析 Dra. Beatriz Cardoso, MSc, ACPV Dr. Oscar Morales, ACPV Lohmann Animal Health 德国罗曼动物保健品有限公司 Serology血清学 What is serology?什么是血清学？ What is it for? 血清学的意义 How to get the best serological results? 怎样获得最佳的血清结果？ How to interpret serology? broilers Layers and breeders 怎样分析血清学结果？ －肉鸡 －蛋鸡和种鸡 Samples and serum banks 采样和血清保存 What is serology?什么是血清学？ Detection of antibodies 抗体的检测 Types of antibodies抗体的类型 IgA IgM IgE IgG Qualitative Tests定性试验 Answer is yes or no 回答“是”或者“不是” Results are “positive” or “negativo” 结果为“阳性”或“阴性” Rapid Plate Agglutination test 快速平板凝集试验 Agar Gel Precipitation test 琼脂凝胶沉淀试验 Semi – Quantitative tests半定量试验 Results expressed in titres 结果用滴度表达 Based on serial dilution 基于连续稀释 Results expressed in 2x, 10x (2log, 10log) 结果表达为2x, 10x (2log, 10log) HI, Serum neutralization HI,血清中和试验 Quantitative tests定量试验 Results in absolute numbers 结果用绝对数表示 Result is the colorimetric reaction of the substrate proportional to the quantitiy of antibodies 结果是与抗体数量成比例的物质的比色反应 ELISA 酶联免疫吸附试验 Sensibility敏感性 Ability to determine minimal quantities of antibodies 敏感性是指能检测到的最小抗体的数量 Avoid false negatives 避免假阴性 False positives 假阳性 Disease control and erradication 用于疾病控制和净化 Example: SPA 举例：SPA Specificity特异性 Antibodies developed against a specific agent 由特异性抗原产生的抗体 Avoid false positives 避免假阳性 False negatives 假阴性 Example: Imnmunofluorescence 举例：免疫荧光试验 Ideal理想状况 Calculations计算 Sensibility: 敏感性 Real Positives/(Total number Infected) x 100 真正的阳性/(合计感染数) x 100 Calculations计算 Especificity: 特异性 Real Negatives/Total number Infected x 100 真正的阴性/合计感染数x 100 Calculations计算 Aplication in the field现场运用 specific test特异性试验 Calculations 2 计算2 Sensibility = 80/(100)x100 = 80% 敏感性＝ 80/(100)x100 = 80% specificity = 100/(100) x 100 = 100% 特异性＝ 100/(100) x 100 = 100% specificity and Sensibility特异性和敏感性 Type of method 方法的类型 Antigen used 使用的抗原 Con