LY294002 Enhances Inhibitory Effect of Gemcitabine on Proliferation of Human Pancreatic Carcinoma PANC-1 Cells研究.pdfVIP

JHuazhongUnivSeiTechnol[MedSei] DOI10．1007／s11596—0131071—5 33(1)：57．62，2013 57 LY294002EnhancesInhibitoryEffectofGemcitabineonProliferationof HumanPancreaticCarcinomaPANC．1Cells Xiao．yuKE(柯晓煜) ，YuWANG(王 渝)，Zuo．qiXIE(谢祚启)。，Zhi—qingLIU(刘志清)，Cui．fangZHANG(张翠芳)， QiuZHAO(赵 秋)，Dong-liangYANG(杨东亮) DepartmentoflnfectiousD~eases，UnionHospital,Tong]fMedicalCollege,HuazhongUniversityofScienceandTechnology, Wuhan430022,China ZDepartmentofGastroenteroloyg,~DepartmentofPathology,Ton~iHospital,TongfiMedicalCollege,HuazhongUniversityof ScienceandTechnology,Wuhan430034China ◎HuazhongUniversityofSciencena dTechnologyandSpringer-VerlagBerlinHeidelberg2013 Summary：Phosphatidylinositide3．kinase(PI3K)p／roteinkinaseBfPKB，Akt1pathwayplaysamajor roleinproli~rmionandsurvivalofmanytypesofcells．TheinhibitoryefrectOfI 294002．widelyap． pliedasaninhibitorofPI3K．incombinationwithgemcitabineonproliferationOfP NC．1cellswas investigated．TheexpressionofPI3K，phosphorylatedAktfp．Akt)nadmultidrng．resistancelikeprotein fMRP1innorrnalpnacreastissues，chronicpancreatitistissuesandpancreaticcarcinomatissueswasde— tected．TheeffectsOfLY294002combinedwithgemcitabineonproliferationofPANC一1cellsandpro— teinlevelsofP．AktnadMRPweredetected．Theresultsshowedthatthepositiveexpressionrateof PI3K．P—AktandMRPinpancreaticcarcinomatissueswassignificantlyhigherthna thatinnormalpan— creastissuesandchronicpancreatitistissues 0．01andP0．05respectively)．LY294002couldeffec— tively enhance theihn ibitory effectofgemcitabineonproliferation ofPANC一1cells．Furthermore，