温湿度处理对Bt棉杀虫蛋白表达的影响.pdfVIP

花 学 报 Cotton Science 20 13 25 渊1冤院63~67 湿度处理对 棉杀虫蛋白表达的影响 1,2 2 2 1 1 1* 永慧 陈建平 蔡立旺 张 祥 陈 源 陈德华 (1.扬州大学江苏省作物遗传生理重点开放实验室 江苏扬州225009 曰2. 江苏沿海地区农业科学研究所/ 农业部沿海盐碱 地农业科学观测试验站, 江苏盐城224401) 摘要院以转 基因抗虫棉杂交种泗杂3 号为试验材料 在人工气候室内设置4 种温度和湿度组合渊高温高湿 度尧高温低湿度尧低温高湿度尧低温低湿度冤 研究了不同生育期温湿度胁迫及胁迫解除后 棉杀虫蛋白表达 量的变化遥 结果表明 温湿度胁迫显著抑制 棉杀虫蛋白的表达遥 同 生育期 高温高湿度下Bt 蛋白含量降 幅最小 胁迫解除后恢复能力最强曰低温低湿度下Bt 蛋白含量降幅最大 恢复能力最弱遥 不同时期温湿度胁迫 下Bt 蛋白含量降低幅度表现为盛铃期 盛花期 盛蕾期 胁迫解除后Bt 蛋白的恢复水平表现出相反的趋 势遥 温湿度胁迫及胁迫解除后Bt 蛋白表达量的恢复与胁迫类型尧棉花生育期密切相关遥 关键词院杀虫蛋白曰温湿度胁迫曰 棉 中图分类号院S562.01 文献标志码院A 文章编号院1002-7807 渊20 13冤01-0063-05 1,2 2 2 1 1 1* WANG Yong-hui , CHEN Jian-ping , CAI Li-wang , ZHANG Xiang , CHEN Yuan , CHEN De-hua (1. 225009, ; 2. 22400 1, ) Using insect-resistant transgenic cotton Sikang 3 as material, four combined stress was desigened to study the effect of combination stress of temperature and humidity and its recovery on insecticidal protein expression of transgenic cotton which was high temperature-humidity, low temperature-humidity, high temperature with low humidity and low temperature with high humidity. The results showed that combined stress decreased insecticidal protein content. Under the same growth period, the de- crease amplitude of Bt content was lowest with high temperature-humidity stress, while highest with low temperature-humidity stress. However, the recovery level of Bt content after released from combined stress was highest with high temperature-humidi- ty stress, while lowest with low temperature-humidity stress. Under the s