滇池中芽孢杆菌的ARDRA分类及溶藻特性-湖泊科学.PDF

See discussions, stats, and author profiles for this publication at: /publication/242178581 ARDRA analysis of Bacillus spp. isolated from Lake Dianchi and their algae-lytic effect Article · anuary 2008 DOI: 10.18307/2008.0518 CITATIONS READS 0 18 5 authors, including: Rongxian Zhang iangsu University 29 PUBLICATIONS 761 CITATIONS SEE PROFILE All content following this page was uploaded by Rongxian Zhang on 15 May 2014. The user has requested enhancement of the downloaded file. J. Lake Sci.(湖泊科学), 2008, 20(5): 675-680 . E-mail: jlakes@ ©2008 by Journal of Lake Sciences 滇池中芽孢杆菌的 ARDRA 分类及溶藻特性* 1 2 3 1 1** 宁 华 , 张荣先, 陈 浩 , 杨志荣 , 孙 群 (1: 四川大学生命科学学院, 成都 610064) (2: 铜仁职业技术学院, 铜仁 554300) (3: 四川省原子能应用研究院, 成都 610066) 摘 要: 针对从滇池中分离到的28 株芽孢杆菌, 用扩增rDNA 限制性酶切片段分析法(ARDRA)进行了初步分类, 共获得5 个 操作分类单元(OUT), 其中 OTU5 为主要类型, 包括 14 株细菌菌株. 同时, 以铜绿微囊藻和水华鱼腥藻为裂解对象, 对该 28 株菌株的溶藻能力进行了测定, 发现OTU5 中有6 株芽孢杆菌具有较好的溶藻能力, 其培养液上清液具有强烈的溶藻作用, 能 在pH7 、25 ℃的条件下抑制受试藻类的生长, 可见, 该6 株芽孢杆菌应是通过分泌胞外物质而起溶藻作用的. 关键词: 芽孢杆菌; ARDRA; 溶藻细菌; 溶藻作用; 滇池 ARDRA analysis of Bacillus spp. isolated from Lake Dianchi and their algae-lytic effect NING Hua 1 2 3 1 1 , ZHANG Rongxian , CHEN Hao , YANG Zhirong SUN Qun (1: College of Life Sciences, Sichuan University, Chengdu 610064, P.R.China) (2: Tongren Polytechnic, Tongren 554300, P.R.China) (3: Sichuan Institute of Atomic Energy, Chengdu 610066, P.R.China) Abstract : A total of 28 Bacillius strains isolated from Lake Dianchi were classified by amplified rDNA restriction analysis (ARDRA) on their 16SrDNA through restriction digestion by Alu I. Our data showed that all strains isolated