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看一篇顶你三个月摸索 套模板让 SCI 飞 提交 我的评论 加载中 已评论 看一篇顶你三个月摸索 套模板让 SCI 飞 SCI 论文不好写，主要障碍在英语上，其实就像以前考四六级考研一样，撰写也是有规律可循，有模版可靠。 Abstract 越简单越好，字数控制在 150～250 words. 具体怎么写呢？先简单描述一下课题的前沿背景，然后引出自己的实验（ In this paper…. or Here, we…）主要陈述实验结果，可稍带提一下所用到的重要方法的名称，然后说明你的结果的意义 These data suggest….；最后总结拔高 In a word…. or In summary…..。 举个栗子： [Background]Acetolactate synthase (ALS) is the key enzyme in isobutanol biosynthetic pathway. Efficient expression of ALS is of great significance for the regulation of isobutanol metabolic pathway. [Methods]The acetolactate synthase gene (alsS) from Bacillus subtilis was amplified by PCR with primers designed according to the sequence of alsS in GeneBank, which is 1 713 bp. Then the alsS was cloned into the expression vector of pET-30a(+). The resulted recombinant plasmid was transformed into Escherichia coli BL2l(DE3) for the overexpression of alsS. [Results]The heterologous expression conditions was optimized to be inducted at an OD600 of 0.6?0.8, 30 °C with 1 mmol/L IPTG for 6 h . ALS was mostly expressed in the supernatant with the activity of 24.4 U/mL, which was improved for 7.13 times. Electrophoretically pure ALS was obtained after HisTrapTMFF affinity chromatography with the specific activity of 95.2 U/mg. [Conclusion]These results contributed to the construction of isobutanol biosynthetic pathway in E. coli. Introduction 描述你所研究课题的研究现状及存在的问题，自然地过度到你的实验意义和重要性。「Although…..there are still… so far」「Here, for the first time, we have used…..」。如果你前面已发过相关的文章，要插入引用一下「We recently showed that…..」或「Our previous studies have shown that… . These results verify that…..」「Here…..」「 To explore this hypothesis, we generated….」. 写好 Introduction 相当于是写综述，需要阅读大量的文献进行梳理总结。这估计不是套个模板就能了事，我们下期再聊。 Materials and Methods 这部分比较简单，基本上书本上都有，比如 PCR、酶活测定等，结合自己的课题做些调整即可，或则直接说具体见某文章或某书。 Results 这一部分比较难写。既要按照一定的逻辑顺序组织内容，又要对实验现象做出解释，还要描述准确、专业。 开头先总结性地概括，然后针对实验结果做点评。描述你的实验操作和看到的结果和表面现象时用过去时态，陈述不结论性的内容时用一般现在时。 描述实验操作常用的短语及句式有: In order to prove…., we used… To demonstrate…., we further… Thu