代谢组学方法在生态毒理学中的应用进展精选.pdfVIP

2011 年 第6 卷 生 态 毒 理 学 报 Vol. 6, 2011 第5 期, 459-465 Asian Journal of Ecotoxicology No. 5, 459-465 梁宇杰, 伍 一军* 中国科学院动物研究所分子毒理学实验室 农业虫害鼠害综合治理研究国家重点实验室, 北京100101 : 代谢组学方法越来越多地用于研究有机体与生态环境的相互作用。近年来, 该方法已被应用于化学物风险评价和野生 动物的疾病诊断, 成为环境科学, 特别是生态毒理学中充满活力的研究方向之一。本文介绍了应用于代谢组学研究的核磁共 振波谱和质谱2 种检测技术, 着重讨论了生态毒理学研究中代谢组学方法在生物标志物的发掘和毒性评价, 以及有机体对环 境影响因子的代谢响应、野生水生动物疾病的诊断和监测等方面的应用。这些代谢组学在生态毒理学领域的应用将促进对 有机体与环境相互作用的认识。 : 代谢组学; 生态毒理学; 毒性评价; 环境胁迫因素; 疾病监测 : 167 -5897(2011) 6-459-07 : X171. 5 : A Progress in Application of Metabonomic Approaches in Ecotoxicology * Liang Yujie, Wu Yijun Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China Received 1 July 2010 accepted 21 July 2011 Abstract: M etabonomics methodology is increasingly being applied to investigate the interactions of organ- isms w ith their natural ecological environment. Recently, this method has been applied to chemical risk as- sessment and diagnosis of diseases in w ild animals, becoming one of the vibrant research areas in environ- mental science and ecological toxicology in particular. T his article introduced tw o detecting and measuring technologies, i. e. , nuclear magnetic resonance spectroscopy and mass spectrometry, w hich are commonly used in metabonomics. T his review focused on the application of metabonomics approach in biomarker de- velopment and risk assessment of toxicants exposure, metabonomic responses to environmental stressors, and disease diagnosis and monitoring of w ild aquatic animals. T his grow ing application of metabolomics in ecotoxicology is believed to shed new light on understanding of important questions related to organism-en- vironment interactions. Keywords: metabonomics; ecotoxicology; toxicity