外源性表面活性物质在分泌性中耳炎动物模型的应用研讨.pdfVIP

哈尔滨医科大学硕士学位论文 中 文 摘 要 目的:探讨表面活性物质和其他药物配伍后对分泌性中耳炎动物模型治疗的效果 比较。 方法:肺表面活性物质的提取;建立豚鼠分泌性中耳炎动物模型，7只经鼓膜注 入表面活性物质;7只经鼓膜注入表面活性物质加地塞米松:8只经鼓膜 注入表面活性物质加青霉素;7只经鼓膜注入表面活性物质加肾上腺素; 7只为空白组;7只为对照组。药物每隔四天施用一次，以声阻抗仪和耳 内窥镜在治疗后的60天内观察。比较分泌性中耳炎动物恢复正常所需天 数。 结果:表面活性物质组在 22.86+1.01天治愈;表面活性物质加地塞米松组在 16.61十。.71天治愈，两者有显著性差异。表面活性物质加青霉素组在 23.13+0.91天治愈，其与表面活性物质组无差别。而其他三组之间无显著 性差异，它们与表面活性物质组相比有极显著性的差异。 结论:表面活性物质可以明显地缓解分泌性中耳炎，与地塞米松配伍后效果更佳。 【关键词】表面活性物质;分泌性中耳炎;咽鼓管 哈尔滨医科大学硕士学位论文 Studyofsurfactantapplyinginlaboratoryanimalsuffering otitismediawitheffusion Abstract Objective:Todetermineandcomparetheeffectofsurfactant aloneandwithothermedicationontheresolutionofexperimentally inducedotitismediawitheffusioninguineapigs.Methods:Toestablish theanimalmodelofguineapigssufferingrfom otitismediawith effusion.Sevenanimalsweretreatedwithtranstympanicinjectionof surfactant;Seven received surfactant with surfactant and dexamethasone;Eightweretreatedwithsurfactantandpenicillin;Seven receivedsurfactantandadrenalin;Sevenreceivednotreatment;Seven weretreatedwithonlyplacebo.Themedicineswereusedeveryfour days,Otomicroscopyandtympanometrywereperformedduring60days aftertreatmentbegan Results: ONE isresolvedafter22.86+1.Oldaysinsurfactant group;Itisresolvedafter16.61+0.71daysinsur-dexgroup.Thereis statisticallysignificantbetweenthem.Nosignificantcouldbefound betweensur一engroupandsurfactantgroup.Theotherthreegroupshave nostatisticallydifferenceeachother;thereisstatisticallysignificant differencebetweenthemandsurfactantgroup. Conclusion:Thestudy demonstratesthatsurfactantcan effectivelydecreasethedurationofOME,itcanbetreatedmore efficaciouslywiththehelpofdexamethasone. keyword:Surfactant;Otitismediawitheffusion;Eustachian tube