正交试验优选麻芎平喘颗粒中药材的提取工艺Δ.PDFVIP

·中药工艺与制剂· 正交试验优选麻芎平喘颗粒中药材的提取工艺Δ 1＊ 1 2 3# 李 卿 ，周 兴 ，欧 燕 ，须 建 （1.重庆市中药研究院，重庆 400065；2.重庆市綦江区人民医院，重庆 401420；3.重庆医药高等专科学校医学技术学院，重庆 401331） 中图分类号 R284.2；R283 文献标志码 A 文章编号 1001-0408（2014）15-1377-03 DOI 10.6039/j.issn. 1001-0408.2014.15.11 摘 要 目的：优选麻芎平喘颗粒中药材的提取工艺。方法：采用正交试验，以盐酸麻黄碱质量分数为评价指标，考察加水量、煎 煮时间和煎煮次数对水提工艺的影响；以盐酸麻黄碱保留率、固形物减少率为评价指标，考察药液相对密度、醇沉浓度、醇沉时间 对醇沉工艺的影响。结果：最佳水提醇沉工艺为加12倍量水，煎煮3次，分别为80、60、60min ，提取液浓缩至相对密度为1.25，冷 却后加入乙醇使含醇量为60％，放置24h ，除去沉淀。结论：优选的水提醇沉工艺稳定、可行，重现性好，可用于麻芎平喘颗粒中药 材的提取。 关键词 麻芎平喘颗粒；正交试验；盐酸麻黄碱；提取工艺 Optimization of the Extraction Technology of Maxiong Pingchuan Granule by Orthogonal Design 1 1 2 3 LI Qing ，ZHOU Xing ，OU Yan ，XU Jian （1.Chongqing Academy of Chinese Materia Medica ，Chongqing 400065，China ；2.Chongqing Qijiang District People ’s Hospital ，Chongqing 401420，China ；3.College of Medi- cal Technology ，Chongqing Medical and Pharmaceutical College ，Chongqing 401331，China ） ABSTRACT OBJECTIVE ：To optimize the extraction technology of Maxiong pingchuan granule. METHODS ：The effects of wa- ter amount ，decoction time and decoction times on the water extraction technology were investigated by orthogonal design ，using the content of ephedrine hydrochloride as index. The effects of relative density ，the concentration and time of alcohol precipitation on alcohol precipitation technology were investigated by using the retention rate of ephedrine hydrochloride and decrement of solid mater as index. RESULTS ：The optimal water extraction-alcohol precipitation technology was as follows ：12folds of water ，decoct- ing 3 times ，extracting for 80，60，60 min. Extract was condensed with relative density of 1.25，and then was added into ethanol after cooling with ethanol content of 60％；the precipitation was removed after placing for 24 h. CONCLUSIONS ：The