百草枯对健康者外周血单个核细胞OPN,NF-KB的影响.docVIP

精品论文 参考文献 百草枯对健康者外周血单个核细胞OPN,NF-KB的影响 张宏华 郝小平 肖芸 　　（粤北人民医院 广东 韶关 512026） 　　【摘要】 目的：探讨百草枯对健康者外周血单个核细胞（peripheral blood mononuclear cell PBMC)骨桥蛋白（osteopontin OPN），核转录因子-KB（Nuclear factor-kB,NF-KB)的影响。方法：健康体检者6例，取外周静脉血分离获得PBMC，以5times;105个细胞/孔种入96孔细胞培养板，分3组培养12小时。A1组：正常PBMC; A2组：正常PBMC＋50ng/ml百草枯；A3组：正常PBMC＋100ng/ml百草枯。观察培养前后细胞形态变化，收集各组细胞培养上清检测骨桥蛋白水平，收获细胞后检测胞内NF-KB。结果：各组细胞培养前后细胞形态、数量无明显变化；从A1组到A3组，细胞培养上清骨桥蛋白水平及胞内NF-KB含量呈逐组增高。结论：百草枯影响细胞内NF-KB，导致骨桥蛋白的大量分泌，可能是其引起肺组织损伤的原因。 　　【关键词】 百草枯；单个核细胞；骨桥蛋白；核转录因子-KB 　　【中图分类号】R969 【文献标识码】A 【文章编号】2095-1752（2016）06-0171-02 　　The synergetic effect of paraquet on osteopontin,Nuclear factor-KB from Peripheral blood mononuclear cells in healthy volunteers 　　 Zhang Honghua,Hao Xiaoping,Xiao Yun.Department of Respiratory Disease,Yuebei Reming Hospital,Shaoguan 512026,China 　　【Abstract】Objective To investigate the synergetic effect of paraquet on osteopontin(OPN), Nuclear factor-kB from peripheral blood mononuclear cell(PBMC) in healthy volunteers. Methods Peripheral blood mononuclear cells from 6 healthy volunteers were obtained after centrifugalization,5times;105PBMCs/well seeded in 96 wells cell culture plate were divided into 3 groups for cultivating 12 hours.A1 group;normal PBMC;A2 group:normal PBMC＋50ng/ml paraquet;A3 group: normal PBMC＋100ng/ml paraquet. Cell morphologic changes before and after culture were observed, OPN of culture supernatant were detected by ELISA, NF-KB within cells were detected by western blot.Results Cell appearance and amount before and after 12 hours culture didnrsquo;t show marked change in three groups;There were obvious difference about levels of OPN within three groups;So was level of NF-KB . Conclusions Paraquet significantly increased OPN of production of PBMC,raised levels of NF-KB within PBMC,maybe gradually induce lung organization damage. 　　【Key words】 Paraquet; Peripheral blood mononuclear cell; Osteopontin; Nuclear factor-kB 　　我们在临床工作中发现，许多百草枯通过皮肤中毒患者，