崔银秋-分子生物学-chapter8 The replication of DNA.pptVIP

CHAPTER 8: The replication of DNA Meselson and Stahl’s Experiment M. Meselson and F.W. Stahl. 1958. The replication of DNA in Escherichia coli Proc. Natl. Acad. Sci. U.S.A. 44: 671-682. M. Meselson and F. W. Stahl were interested in trying to devise a way to prove or disprove Watson and Cricks model of semi-conservative replication. In 1957, they successfully obtained the experimental proof for the semi-conservative replication of DNA. They did this by inventing a new technique called Density Gradient Centrifugation. Their paper was published in 1958 and ever since, the experiment has often been referred to as: one of the most beautiful experiments in biology. A. The Meselson-Stall experiment B. the interpretation Complications of DNA replication Enzymes The Topological Problem Direction problem: semi-discontinuously Priming The Chemistry of DNA Synthesis DNA Polymerase The Replication Fork The Replication Process Raw materials Reaction direction? Chemical reaction? The driving force for DNA synthesis Diagram of the mechanism of DNA synthesis DNA Polymerase The specialization of DNA polymerases DNA polymerases of bacteria The composition of the DNA Pol III holoenzyme Sliding clamps Encircle the newly synthesized double-stranded DNA and the polymerase associated with the primer:template junction Ensures the rapid rebinding of DNA Pol to the same primer:template junction, and thus increases the processivity of Pol. Eukaryotic sliding DNA clamp is PCNA Sliding DNA Clamp Increases Processivity Polymerase switching DNA Polymerase The specialization of DNA polymerases DNA Polymerase-palm domain DNA Polymerase The specialization of DNA polymerases DNA rapid processive synthesis DNA accurate synthesis The Chemistry of DNA Synthesis DNA Polymerase The Replication Fork The Replication Process The replication fork The junction between the newly separated template strands and the unreplicated duplex DNA Mechanism Of DNA Chain Growth, II. Accu