多酶复配优化木质纤维素酶解工艺研讨.pdfVIP

研究论文 多酶复配优化木质纤维素酶解工艺研究 张名佳 苏荣欣 齐崴 何志敏* （天津大学化工学院化学工程研究所，天津，300072 ） 摘要：本文利用多酶复配的方法酶解氨水预处理后的玉米芯，以提高纤维素和半纤维素转化率。实验确定了纤 维素酶和β-葡萄糖苷酶的饱和添加量，分别为每克葡聚糖50 FPU 和60 CBU 。每克葡聚糖添加30 CBU β-葡萄糖 苷酶即可及时水解纤维二糖，消除其抑制作用。在酶解体系中添加木聚糖酶和果胶酶，可水解包裹在纤维素表面 的半纤维素及果胶。实验发现：加入复配酶——木聚糖酶和果胶酶后，均有利于纤维素和半纤维素的转化，且木 聚糖转化率提高明显。当每克纤维素加入0.67 mg 木聚糖酶蛋白时，纤维素转化率提高了8%，木聚糖水解效率 提高 1.7 倍；添加少量果胶酶（每克葡聚糖0.12 mg 果胶酶蛋白）时，可使纤维素转化率提高7% ，木聚糖转化 率提高21.3% 。 关键词：木质纤维素；纤维素酶；β-葡萄糖苷酶；木聚糖酶；果胶酶 中图分类号：TK6 ；S216.2 文献标识码：A 文章编号：0438-1157 （2008 ）00-0000-00 Enhance enzymatic hydrolysis of corncob by enzyme complexes * ZHANG Mingjia ，SU Rongxin，QI Wei，HE Zhimin (School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China) Abstract ：In order to enhance the conversion of the cellulose and hemicellulose, the corncob pretreated by aqueous ammonia was enzymatic hydrolyzed by enzyme complexes. The saturation limits for cellulase and -1 -1 β-glucosidase was determined, which was 50 FPU·g (glucan) and 60 CBU·g (glucan), respectively. 30 -1 CBU·g (glucan) was enough to eliminate the cellobiose inhibitor. Xylanase and Pectinase can hydrolyze the hemicellulose and pectin components of lignocellulose which cover the fibre surfaces. Supplemented these two accessory enzymes can increase the conversion of cellulose and hemicellulose significantly. Addition of 0.67 mg -1 protein·g (glucan) increased the cellulose conversion by 8%, and 1.7-fold in xylan conversion. When