乳酸菌学术交流.ppt

Protein Profile and Plasmid Content of  LL52 and LC79 Strainsunder Several Stress Conditions Seminar 2008-5-22 Introduction stress conditions industrial processing low pH, the absence of nutrients, unfavorable osmotic conditions, high or low temperatures Lactococcus lactis important: dairy foods and nisin Introduction 2 L. lactis strains L. lactis subsp. lactis LL52 L. lactis subsp. cremoris LC79 stress conditions high and low temperature, high salt, and low pH plasmid and protein content , , s s L. lactis subsp. lactis LL52 L. lactis subsp. cremoris LC79 M17 broth M17 broth；30℃；pH 7.4 Bacterial stocks： M17 broth；–18℃ Bacterial Survival under Stress Conditions Protein Extraction and Analysis Plasmid DNA Isolation and Analysis survival rate under stress conditions Both strains were tolerant to: high temperature low temperature low pH Bile salts treatment: first 30min: drop drastically 30-90min: remain constant result result Discussion response of 2 strains to different stress conditions produce some novel proteins / increase production of already existing proteins inhibit / decrease production of some proteins speculate: most probably the result of protein modification or gene regulation confirm：examine the proteins at the molecular and biochemical levels starter culture strain * * * * * Lactococcus lactis subsp. lactis Lactococcus lactis subsp. cremoris 乳酸乳球菌 乳脂亚种 乳酸乳球菌 乳 亚种 * / 15 Materials and Methods Results Discussion Introduction Contents Growth Conditions Media Bacterial Strains stress conditions plated onto M17glu medium dispensed into 4 tubes incubation collect sample count CFU1 centrifugate resuspend remove sample dilute count CFU2 survival rate 5ml, M17glu medium, 30℃, stationary phase cell pellets 10ml M17glu medium 30min, 90min 0.9%NaCl 0.1m