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Signal Transduction Cascade for Regulation of RpoS…
JOURNAL OF BACTERIOLOGY, July 2001, p. 4012–4023 Vol. 183, No. 13
0021-9193/01/$04.00 0 DOI: 10.1128/JB.183.13.4012–4023.2001
Signal Transduction Cascade for Regulation of RpoS:
Temperature Regulation of DsrA
F. REPOILA AND S. GOTTESMAN*
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health,
Bethesda, Maryland 20892-4255
Received 7 March 2001/Accepted 10 April 2001
Many environmental parameters modulate the amount of the RpoS sigma factor in Escherichia coli . Tem-
perature control of RpoS depends on the untranslated RNA DsrA. DsrA activates RpoS translation by pairing
with the leader of the mRNA. We find that temperature affects both the rate of transcription initiation of the
dsrA gene and the stability of DsrA RNA. Both are increased at low temperature (25°C) compared to 37 or 42°C. D
The combination of these results is 25-fold-less DsrA at 37°C and 30-fold less at 42°C than at 25°C. Using an o
w
n
adapted lacZ-based reporter system, we show that temperature control of transcription initiation of dsrA
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