大环内酯类药物对金黄色葡萄球菌的防耐药突变-第三军医大学学报.DOCVIP

大环内酯类药物对肺炎链球菌的防耐药突变浓度的 邢 茂1，刘同华1，王琴2（1第三军医大学新桥医院药剂科，重庆 4000372重庆市中医研究院，重庆 400013） 摘 要： 目的 测定大环内酯类药物对肺炎链球菌的防耐药突变浓度（MPC），扩增突变选择窗(mutant selection window MSW)内筛选的耐药突变体的耐药基因，以了解肺炎链球菌对大环内酯类药物耐药的发生机制（问题2：目的重写。）。 方法 肉汤法富集1010 CFU/ml ATCC49619，采用平板二倍稀释法测定罗红霉素和阿奇霉素对ATCC49619的最低抑菌浓度（minimal inhibitory concentration, MIC）、MIC99、初测MPC(Provisional MPC，MPCpr)以及MPC。PCR法扩增不同药物筛选出的耐药突变株的耐药基因ermB 和mefA并测序。 结果 罗红霉素和阿奇霉素对ATCC49619的MPC分别为0.80 μg/ml和0.51 μg/ml, 细菌耐药选择指数（MPC/ MIC99）为5.0和3.9。筛选出的耐药突变株中扩增出ermB耐药基因。结论 通过调整药物剂量，可以限制对大环内酯类耐药的肺炎链球菌突变体的富集。肺炎链球菌对大环内酯类药物耐药。 关键词防耐药变异浓度Study of mutant prevention concentrations of macrolides for Streptococcus pneumoniae XING Mao1 , LIU Tong-hua1, Wang Qin2（1Department of Pharmacy, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China；2 Chongqing Institute of Traditional Chinese Medicine, Chongqing 400013,China） Abstract：Objective To measure mutant prevention concentrations ( MPC) of Macrolides for Streptococcus pneumoniae and Amplify of resistance gene of Streptococcus pneumoniae mutants selected in the mutant selection window(MSW).To investigate the mechanism of Streptococcus pneumoniae resistance to Macrolides. Methods The Streptococcus pneumoniae strain ATCC49619 were enriched in broth, and the bacterial concentrations were adjusted to 1010 colony forming units per milliliter. The minimal inhibitory concentration (MIC) , MIC for 99 % of input cells(MIC99) , provisional MPC ( MPCpr ) and MPC of roxithromycin and azithromycin for Streptococcus pneumoniae were determined by agar plates dilution method. The ermB and mefA of Streptococcus pneumoniae mutants selected in the MSW were obtained by PCR method and sequencing. Results The MPC of roxithromycin and azithromycin for ATCC49619 were 0.80 μg/ml and 0.51 μg/ml, the MPC/ MIC99 were 5.0 and 3.9. The ermB gene were detected in Streptococcus pneumoniae mutants selected in the MSW. Conclusion By adjusting the drug dose, may limit the enrichment of macrolide-resistant St