酶催化合成蔗糖酯研究进展-河南农业科学.pdfVIP

546 2007, Vol. 28, No. 07 食品科学 ※专题论述 1 2 2, 邹义英 ，谭贤勇 ，李军生 * (1.广西工学院图书馆，广西 柳州 545006；2. 广西工学院生物与化学工程系，广西 柳州 545006) ：本文对蔗糖酯的酶促合成研究进行了总结，其中主要涉及到酶、反应媒介和酰基供体等影响因素。研 究发现，蛋白酶、脂肪酶和抗体酶均能区域选择地酰化蔗糖。酶催化合成蔗糖酯通常在N,N-二甲基甲酰胺(DMF)， 二甲基亚砜(DMSO)和吡啶等有毒溶剂中进行。近几年来，使用混合溶剂或叔丁醇等毒性较小的溶剂代替毒性较大 的溶剂，以及无溶剂条件下酶催化合成蔗糖酯的研究，也越来越受重视。活化酯作为酰基供体得到广泛的应用。 ：蔗糖酯；酶催化合成；溶剂；酰基供体 Research Progress of Enzyme-catalyzed Synthesis of Sucrose Esters 1 2 2, ZOU Yi-ying ，TAN Xian-yong ，LI Jun-sheng * (1.Library of Guangxi Universty of Technology, Liuzhou 545006, China； 2.Department of Biological and Chemical Engineering, Guangxi University of Technology, Liuzhou 545006, China) Abstract ：This article reviews the recent development in the sucrose esters synthesis catalyzed by enzymes, namely those involving control over the enzyme, the reaction medium and the acyl donor. It was discovered that proteases, lipases and abzymes can be used for the regioselective acylation of sucrose. Enzymatic regioselective synthesis of sucrose ester is com- monly carried out in dimethylformamide(DMF), dimethylsulfoxide(DMSO), pyridine and other solvents which are poisonous. In recent years, the studies on enzyme-catalyzed regioselective synthesis under solvent-free conditions and on processes of the use of less harmful solvents such as mixtures of miscible solvents or tert-butanol have been paid more attention. The activated acyl donor widely. esters have been used as the Key words：sucrose ester；enzymatic synthesis；solvent；acyl donor 中图分类号：TQ426.97 文献标识码：A