TLR在介导嗜酸乳杆菌预防雏鸡UC发生中的作用及-中国科技论文在线.PDFVIP

中国科技论文在线 TLR 在介导嗜酸乳杆菌预防雏鸡UC 发生 中的作用及其机制 # 刘晓静，孙正阳，郑世民，高雪丽，刘超男** 5 （东北农业大学动物医学学院，哈尔滨150030 ） 摘要：为了探索TLR 在嗜酸乳杆菌预防UC 发生中的作用及其机制，将75 只1 日龄SPF 雏 鸡随机分成正常对照组、结肠炎模型组和嗜酸乳杆菌预处理组3 组，采用荧光定量PCR 方 法检测结肠组织TLR2 和MyD88 mRNA 表达量，ELISA 方法检测血清中IL-8 和IL-10 含量 10 以及采用HE 染色的方法观察雏鸡结肠组织病理学变化。结果显示，嗜酸乳杆菌预处理组雏 鸡结肠组织TLR2、MyD88mRNA 表达以及血清中IL-8 含量均极显著 （P0.01 ）低于结肠炎 模型组，显著 （P0.05 ）高于对照组雏鸡，血清中IL-10 含量极显著高于结肠炎模型组和正 常对照组。研究表明，嗜酸乳杆菌可通过TLR2/MyD88 信号通路介导血清中IL-8 和 IL-10 的含量，缓解雏鸡UC 的发生和发展。 15 关键词：基础兽医学；嗜酸乳杆菌；溃疡性结肠炎；TLR ；细胞因子 中图分类号：S852.3 The Role of TLR in Lactobacillus Acidophilus Preventing the Occurrence of Ulcerative Colitis in chickens 20 LIU Xiaojing, SUN Zhengyang, ZHENG Shimin, GAO Xueli, LIU Chaonan (Veterinary Medical School,Northeast Agricultural University,Harbin 150030) Abstract : In order to explore the role and mechanism of Lactobacillus acidophilus in preventing the occurrence of UC, 75 one-day old SPF chickens were randomly divided into control group, model group and Lactobacillus acidophilus pretreatment group. The expression of TLR2 and MyD88 in colon tissues 25 was detected by Real Time PCR method. The IL-8 and IL-10 contents in serum were detected by ELISA method and the histopathological changes of the colon were observed by HE staining method. The results showed that Lactobacillus acidophilus pretrearment group chickens colon tissue TLR2, MyD88mRNA expression and serum IL-8 levels were significantly lower (p0.01) than colitis model group chickens, significantly higher (p0.05) than the control group chickens. The content of IL-10 in serum was 30