沉默ABCG2基因对人肺腺癌细胞GLC82体内增殖的影响研究.pdf

沉默ABC62基因对人肺腺癌细胞GLC-82体内增殖的影响 曾媛牛海艳中洪 南方医科大学病理教研室，广东广 摘要： 目的探讨ABCG2基冈沉默对人肺腺癌细胞GLC．82体内增殖的影响。方法将人肺腺癌 内的增殖。 关键词肺癌；ABCG2；Ki．67；核仁组成区；图像分析 the invivo EffectofABCG2 on genesilencing ability proliferative cell． ofhumancarcinoma lung ZENG Yuan，NIUHai-yang，SHENHong ofSouthernMedical 510515，China PathologyDepartment University,Guan97_hou Abstract To the on invivoof observeeffectofABCG2 0bjective genesilencingproliferativeability humancarcinomacell．MethodsHumancarcinomacellsGLC．82．enhancedfluorescence lung lung green humancarcinomacellsGLC．82，EGFP+／ABCG2一and protein(EGFP)．1abeledlung intosubcutaneousof tumorswereexamined implanted tissuenudemice．The by Ki．67．ABCG2and methodsfollowed using AgNOR byquantitativecomputerizedimageanalysis．Results TheKi．67 iferationindexofGLC．82／EGFP+^～BCG2cellsinvivowas lowerthan prol significantly GLC．82／EGFP+cellsandGLC．82 wellasthe ofABCG2 cells(P0．05)，asexpression protein．Image revealed differencesin withlessernumberof andtotal analysis significant AgNORparameters AgNOR areasbut mean areasinthecellsof AgNOR higherAgNOR the of in ABCG2 call carcinomacellvivo． g