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DYNALogue 02/2002 Customer Report CD4+CD25+ Introduction: Biotech). Those cells not selected by the Dynabeads® on There is substantial evidence that immune regulation the magnet (Dynal MPC®) were designated CD4+CD25-. cells isolated by plays an important part in tolerance to self-antigens and The beads were then washed four times and the ® Dynabeads alloantigens in experimental models of autoimmune supernatants discarded. The CD4+CD25+ cells were display disease and transplantation [1]. The discovery that the released from the Dynabeads® by mixing with CD4+ T cell is essential in this process [2] has focused DETACHaBEAD® CD4/CD8 (Dynal Biotech) at 10 ml 7 classical ® attention on identifying T cell subsets that are DETACHaBEAD in 100 ml medium per 10 cells at 20°C for regulatory responsible for this effect. Since 1995 there has been 1 hour. The beads were then removed with a Dynal MPC®.