HCY国际参考物质精选.pdfVIP

Anal Bioanal Chem DOI 10.1007/s00216-006-0434-1 ORIGINAL PAPER Mary B. Satterfield . Lorna T. Sniegoski . Katherine E. Sharpless . Michael J. Welch . Adriana Hornikova . Nien-Fan Zhang . Christine M. Pfeiffer . Zia Fazili . Mindy Zhang . Bryant C. Nelson Development of a new standard reference material: SRM 1955 (homocysteine and folate in human serum) Received: 31 December 2005 / Revised: 14 March 2006 / Accepted: 16 March 2006 # Springer-Verlag 2006 Abstract Total homocysteine (tHCY) and folate are recognized certified reference materials, as described by interrelated biomarkers for arteriosclerosis and coronary European Directive 98/79/EC on in vitro diagnostic heart disease. Although many different methods for both medical devices. tHCY and folate are clinically available, the intermethod and interlaboratory results are often poor, resulting in the Keywords Isotope dilution mass spectrometry . need for a matrix reference material and reference methods. Standard reference material . Homocysteine . Folate . The National Institute of Standards and Technology (NIST) 5-Methyltetrahydrofolic acid . 5-Formyltetrahydrofolic has developed isotope dilution liquid chromatography/ acid . Folic acid mass spectrometry (LC/MS) and liquid chromatography/ tandem mass spectrometry (LC/MS/MS) methods for determination of tHCY and several folate forms including Introduction 5-methyltetrahydrofolic acid (5MT) and folic acid (FA). Additionally, a method for simultaneous measurement of Total homocysteine (tHCY) and folate are markers related tHCY, 5MT, and FA has been developed and validated. In to cardiovascular health that have been targeted by the collaboration with the Centers for Disease Control and National Institute