细胞信号转导文献3.pdfVIP

133 Focal adhesions – the cytoskeletal connection David R Critchley • Cellular contacts with the extracellular matrix are regulated a higher affinity than β1A for talin molecules [4 ], consis- by the Rho family of GTPases through their effects on both tent with the idea that tight binding of β1D to talin in the actin and the microtubule cytoarchitecture. Recent myotendinous junctions enables this structure to with- genetic, biochemical and structural data have highlighted stand high tensile forces. the role played by a subset of actin-binding proteins in coupling integrins to cytoskeletal actin and in assembling Talin contains a well-characterised actin-binding site close signalling complexes that are important for cell motility and to its carboxy-terminal region [8,9], which shows homology cell proliferation. with the yeast actin-binding protein Sla2p and the hunt- ingtin-interacting protein Hip1 [10]. Preliminary evidence Addresses for two other actin-binding sites in talin has also been pre- Department of Biochemistry, University of Leicester, University Road, sented [8]. Actin binding is optimal at low salt Leicester LE1 7RH, England; e-mail: drc@leicester.ac.uk concentrations and at pH 6.4, the significance of which Current Opinion in Cell Bi