腺苷预处理对糖氧剥夺损伤星形胶质细胞的影响.docVIP

精品论文 参考文献 腺苷预处理对糖氧剥夺损伤星形胶质细胞的影响 韩艳艳 栗延伟 谭军 尉娜（通讯作者） 　　（新乡医学院第三附属医院神经内二科 河南新乡 453003） 　　【摘要】目的：观察糖氧剥夺对星形胶质细胞（Astrocyte，Ast）的影响及腺苷的保护作用。方法：体外培养SD大鼠大脑皮层Ast，培养至第四代时传代至96孔板内，待细胞贴壁并融合、长出突起后，随机分为三组：正常对照组(normal control group)、糖氧剥夺组（oxygen glucose deprivation group, OGD group）、腺苷预处理组（adenosine group, ADO group），进行糖氧剥夺/复氧糖处理。观察糖氧剥夺8h复氧糖24h后Ast的细胞活率及培养液中乳酸脱氢酶（lactate dehydrogenase, LDH）的浓度。结果：OGD组Ast活性明显低于对照组，LDH浓度高于对照组；ADO组细胞活率明显高于OGD组，LDH浓度低于OGD组（P＜0.01）；3组间两两比较细胞活率及细胞培养液中LDH浓度差异有统计学意义（P＜0.01）。结论：腺苷能减少糖氧剥夺Ast的损伤，减少糖氧剥夺后Ast凋亡，腺苷预处理对缺糖缺氧Ast有一定的影响。 　　【关键词】腺苷 缺血预处理 糖氧剥夺 星形胶质细胞 乳酸脱氢酶 　　【中图分类号】R473 【文献标识码】A 【文章编号】2095-1752（2014）16-0078-02 　　The Influence of Adenosine Preconditioning on Oxygen-glucose deprivation injury in vitro astrocyte cells 　　【Abstract】 Objective To investigate the effect of oxygen-glucose deprivation and protective effect of adenosine on astrocytes. Methods Astrocytes were cultured for fourth generation in vitro, and then passaged at 96 well plate. When the astrocytes attaching to the wall and growing with prominences, they were randomly divided into 3 groups, normal control group, oxygen-glucose deprivation (OGD) group and adenosine preconditioning (ADO) group. The cells were taken OGD/reoxygenation treatment. The concentration of LDH of astrocyte cells were observed at 8 hours after OGD and 24 hours after reoxygenation. Results The cell viability in the OGD group and ADO group produced a significant decrease. the viability in ADO group were higher than that of OGD group(Plt;0.01). Compared with the normal group, the LDH concentration in OGD group was significantly increased, compared with OGD group, LDH concentration in ADO group obviously decreased (Plt;0.01). There were significant differences between each two groups (all Plt;0.01). Conclusions Adenosine could reduce the injury and apoptosis of oxygen-glucose deprivation, and we can conclusion that adenosine has influence on astrocytes after