读书报告12磷酸对天竺葵青枯病的控制PPT.pptVIP

Control of Bacterial Wilt of Geranium with Phosphorous Acid  D. J. Norman 磷酸对天竺葵青枯病的控制;主要内容：;一、前言;Geraniums are susceptible to races 1 and 3 of R. solanacearum . Most geraniums produced in the world are vegetatively propagated in Guatemala, Costa Rica,Columbia, China, and Kenya. In all these locations, endemic populations of R. solanacearum exist . There are no known treatments that are effective in protecting geranium plants. 天竺葵对于青枯菌属1和3是敏感的，天竺葵广泛种植在世界各地如危地马拉，哥斯达黎加，哥伦比亚，中国和肯尼亚。但这些地方都存在地方性青枯病，目前还没有有效的方法保护天竺葵作物。 Thus, the objective of this research was to determine if geranium plants could be protected from infection with applications of selected bactericides and chemicals. 因此本实验的目的就是对天竺葵作物施以筛选过的细菌和化学药品来验证是否能保护作物免受侵染。;二、材料与方法1 Screening of products. ; Two additional treatments were included in each test: a noninoculated control (saline, 8.5 g NaCl/liter applied without R. solanacearum) and a disease control (no product applied, inoculated with pathogen). 每个实验增加两个处理：不接种控制（含有盐分，NaCl，无青枯菌）;病害控制（不施其他物质，但接种病菌）。 For inoculum production, R. solanacearum strains were grown on triphenyltetrazolium chloride (TZC) medium (11) for 48 h, and cells were harvested and spectrophotometrically adjusted (A600) in saline to 5 × 108CFU/ml. 接种青枯菌株的要在氯化三苯基四氮唑（TZC）介质中生长48小时， 达到5 × 108CFU/ml.的时候收获菌株。; Wilt symptoms were recorded as they occurred. Bacteria were reisolated 2 weeks after last treatment (6 weeks after inoculation) from plants without wilt symptoms. A cross-section of stem 1 cm in length was removed approximately 0.5 cm above the soil line of each geranium plant. 萎蔫症状一出现就要记录下来，最后一次处理之后两周，细菌就要从没有萎蔫症状的植物中分离。取1cm茎，从离地面0.5cm开始取。通过处理把悬浮液加到TZC介质中培养然后进行检测。 2 Further testing of promising products. Geranium plants were found not to be systemically infected by R. solanacearum when treated with either K-Phite (a.i. 53% m