四种新型合成的CpG寡脱氧核苷酸对淡水长脚 大虾的免疫调节特性.pdfVIP

Open Journal of Fisheries Research 水产研究, 2017, 4(4), 166-179 Published Online December 2017 in Hans. /journal/ojfr /10.12677/ojfr.2017.44024 Immunoregulatory Characteristics of Four Novel Synthetic CpG Oligodeoxynucleotides for Freshwater Giant Prawn (Macrobrachium rosenbergii) * Hung-Hung Sung , Yin-Han Yang, Wei-Xuan Lai Department of Microbiology, Soochow University, Taipei Taiwan th th th Received: Dec. 4 , 2017; accepted: Dec. 19 , 2017; published: Dec. 29 , 2017 Abstract Different sequences of CpG oligodeoxynucleotides (CpG-ODNs) have been demonstrated to result in different expressions of prophenoloxidase gene (propo) in freshwater giant prawn (Macrobra- chium rosenbergii). In this study, in order to assess the possible applications of CpG-ODNs with different immunomodulatory effects that may be used in aquaculture, four novel CpG-ODNs (ODN3006, ODN4006, ODN2050 and ODN2051), which have been designed according to the dif- ferent sequences of CpG-ODNs that promote or inhibit human and fish immunity, are used to de- termine their effect on prawn immunity. First, the binding rate of ODN to hemocytes analyzed by flow cytometry showed that the lowest concentration of ODN2005 required to binding 50% he- mocytes (BC50) was at 1.88 μg/mL and that the concentration was at 5 μg/mL of ODN2050; it could bind more than 90% hemocytes. After hemocytes treated with different concentrations of ODN in vitro, all four ODNs shown increase the extracellular total PO activity (POT) secreted from hemo- cytes, OND2050 at 0.67 μg/mL which not only significantly enhanced POT (p 0.01), but also