酿酒酵母细胞中内质网应激与未折叠蛋白反应的研究进展1.PDFVIP

酿酒酵母细胞中内质网应激与未折叠蛋白反应的研究进展1 赵运英，王頔，袁凡，蒋伶活* (江南大学 生物工程学院, 粮食发酵工艺与技术国家工程实验室, 中国江苏 无锡 214122) 摘 要: 内质网应激激活的未折叠蛋白反应（Unfolded protein response，UPR ）途径在酿酒酵 母和哺乳动物细胞中是非常保守的。内质网（Endoplasmic reticulum ，ER ）是蛋白质合成、 折叠和修饰的细胞器，也是贮存Ca2+ 的主要场所之一。酵母细胞内质网Ca2+平衡与UPR 的 作用是相互的；两个MAPK 途径-HOG 途径和CWI 途径都是细胞应答内质网应激压力时生 存所必须的；重金属镉离子能够激活UPR 途径，它通过激活钙离子通道Cch1/Mid1 进入细 胞影响钙离子的功能。本文结合最新研究进展对酿酒酵母细胞中的两个MAPK 途径、镉离 子和钙离子稳态与内质网应激激活的UPR 途径之间相互关系进行综述。 关键词: 未折叠蛋白应答；内质网应激；MAPK ；钙离子信号途径；酿酒酵母 中图分类号Q93 文献标识码A 文章编号 Endoplasmic Reticulum Stress and Unfolded Protein Response in Saccharomyces Cerevisiae ZHAO Yun-ying, WANG Di, YUAN Fan, JIANG Ling-huo* (The National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China) Abstract: Unfolded protein response (UPR) signaling pathway activated by endoplasmic reticulum stress is highly conserved in both Saccharomyces cerevisiae and mammalian cells. Reticulum Endoplasmic (ER) is a organelle for protein synthesis, folding and modification as well 2+ 2+ as one of the main places for storage Ca . The homeostasis of Ca and UPR are interrelated and interact on each other. The two MAPK pathways, HOG pathway and CWI pathway are all necessary for cell survival under ER stress treated conditions. And cadmium is also able to activate the UPR pathway and it enters into the cells through activating the calcium channel Cch1/Mid1 to affect the function of calcium ion. The interactions between two MAP kinase pathways, cadmium or calcium ion homeostasis and UPR signaling activated by endoplasmic reticulum stress