【精品论文】西贡蕉枯萎病生防木霉菌株gz_2的鉴定及生物学特性研究.pdf

2010 23 5 西 南 农 业 学 报 Vol23 No5 Southwest China Journal of gricultural Sciences 1533 : 1001- 4829( 2010) 05- 1533- 07 gz2 1 2* 3 1 4 1 柯仿钢 , 黄思良 , 付 岗 , 汪 茜, 张 欣 , 黎起秦 ( 1. , 530005; 2. , 473061; 3. , 530007; 4. , 571737) : 拮抗西贡蕉枯萎病菌的木霉菌株 gz2 是一株具有生防潜力的拮抗菌, 依据其形态特征和 ITS序列 其鉴定为哈茨木霉 (T richoderm a harz ianum R ifai)gz2菌株的生物学特性测定结果表明, 该菌株最适宜在马铃薯蔗糖琼脂(PS )培养基上生长; 蔗 糖酵母膏分别为最佳碳氮源; 菌丝最适生长温度范围为 28~ 30 ; 最适 pH 5. 0~ 6. 0; 不同光照条件对该菌产孢影响明显对西 贡蕉枯萎病的盆栽防治试验结果表明, gz2菌株对西贡蕉枯萎病具有一定的防效, 其中每隔 7 d施 1次 gz2固体菌剂处理的防治 效果最好, 在伤根接种 60 d后, 平均防效达到 74. 4% : 西贡蕉; 枯萎病; 木霉菌株 gz2; 生物学特性; 防治效果 : S688. 1 : Identification and Biological Characteristics of Trichod erma Strain gz2, A B iocontrolAgent against Saigon Banana Fusarium W ilt 1 2* 3 1 4 1 KE Fanggang , HU NG Siliang , FU G ang , W NG Q ian , ZH NG X in , LIQi qin ( 1. griculturalCollege, GuangxiUniversity, NanningGuangxi530005, China; 2. NanyangNormalUniversity, N anyangHenan 473061, China; 3. M icrobiology Research Institute, Guangxi cademy of griculturalSciences, NanningGuangxi 530007, China; 4. Environment and Plant Protection Institute, Chinese cademy of Tropical gricultural Sciences, DanzhouHainan 571737, China) A bstract: Trichoderm a strain gz2 is a potential biocontrol agent against Fusarium w ilt of Saigon banana. The strain was identified as Tri chod erma harz ianum R ifaibased on itsmorphological characteristics and sequence analysis of the internal transcribed spacer region of the ri bosomal DN ( ITS). The biological characteristics of strain gz