Quawell超微量分光光度计.pptVIP

Sample Type: 1Abs =1mg/ml, BSA, IgG, and Lysozyme Dye1 and Dye 2 Selection Windows 1. Dye1 or Dye2 drop-down list: displays the dye that is pre-predefined using the Dye List Editor. 2. Abs: absorbance of Dye1 or Dye2. 3. uM: concentration of Dye1 or Dye2 in uM. 4. Vertical Lines: the green line on the absorbance-wavelength graph indicates the peak position of the wavelength for Dye 1, and the blue vertical line indicates the peak position of the wavelength for Dye 2. Example5:UV-VIS MEASUREMENT The Q5000 can function as a general-use laboratory spectrophotometer. The UV-Vis measurement module provides the operator with a sample absorbance range from 220 to 850 nm. Q5000 是一款功能强大的实验室分光光度计。 UV-Vis measurement 模式可以在220-850nm的波长范围内对样品的吸光值进行测量。 (λ1) nm: select the wavelength by using the up/down arrows. Abs 1 (1mm): corresponding absorbance at (λ1) nm. (λ2) nm: select the wavelength by using the up/down arrows. Abs 2 (1mm): corresponding absorbance at (λ2) nm. λ1 和λ2设定需要测定吸光值的波长位置。 Baseline: if selected, the absorbance value of the baseline is subtracted from the absorbance of Abs1 and Abs2. 如果选择此项，则基准线处吸光值将从Abs1 和Abs2中去除。 Vertical Lines: the green line on the absorbance-wavelength graph indicates the peak position of the wavelength for (λ1)nm, and the blue vertical line indicates the peak position of the wavelength for (λ2)nm. Example5: CELL CULTURES The Q5000 allows laboratories to monitor the density of suspended cell and microbial cultures by measuring their light scatter at 600 nm. Q5000 可以通过检测600nm处的光散射情况来观察悬浮细胞或微生物培养物的密度。 Baseline: if selected, the absorbance value of the baseline is subtracted from the absorbance at 600nm. Selected nm: user select wavelength. Abs (1mm): absorbance at selected nm. Green vertical line: indicates the peak position of the wavelength for selected nm. THE PREDEFINED FLUORESCENT DYES LIST The list works with both the Microarray and Labeled Proteins modules. This list contains a predef