灵芝子实体中两个新的天然三萜类化学成分的分离_纯化和鉴定.pdfVIP

菌 物 学 报 25 （4 ）：599~602, 2006 Mycosystema 灵芝子实体中两个新的天然三萜类化学成分的分离、 纯化和鉴定 1，2 2* 2 3 3 4* 郝瑞霞 张劲松 唐庆九 胡立宏 郑宗平 潘迎捷 1 2 3 （南京农业大学 生命科学学院，南京210095 ； 上海市农业科学院 食用菌研究所，上海201106 ； 中国科学院 上海 4 药物研究所，上海201203 ； 上海水产大学 食品科学学院，上海200090 ） 摘 要：本文采用硅胶和MCI 柱层析的方法，从灵芝Ganoderma lucidum 子实体中分离纯化三萜类化合物。 从灵芝子实体的氯仿萃取层中，分离纯化到灵芝属中的2 个新天然产物，运用现代NMR 技术分析确定了 它们的结构，分别为methyl 7β- hydroxy-3, 11, 15, 23-tetraoxo-5α-lanost-8-en-26-oate(methyl ganoderate D)( Ⅰ) 和methyl 12β-acetoxy-3,7,11,15-tetraoxo-5α-lanost-8-en-24-oate(methyl lucidenate D) ( Ⅱ) 。 关键词：生物活性成分，核磁共振，化学结构 中图分类号：Q939.96 文献标识码：A 文章编号：1672-6472 （2006 ）04-0599-0602 Isolation, purification and identification of two new triterpenoid constituents from the fruiting bodies of Ganoderma lucidum HAO Rui-Xia1, 2 ZHANG Jin-Song2 TANG Qing-Jiu2 HU Li-Hong3 ZHENG Zong-Ping3 PAN Ying-Jie4 (1 College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 3 Shanghai Institute of Meteria Medica, Chinese Academy of Sciences, Shanghai 201203, China; 4 College of Food Science, Shanghai Fisheries University, Shanghai 200090, China) Abstract: The triterpenoid constituents from the fruiting bodies of Ganoderma lucidum were isolated and purified by means of column chromatography. Two new natural products from Ganoderma fruiting bodies were obtained from the chloroform extract. Their structures were identified as methyl 7 β-hydroxy-3, 11, 15, 23- tetraoxo-5α-lanost-8-en-26-oate （methyl ganoderate D ）(I) and methyl 12β-acetoxy- 3, 7, 11, 15- tetraoxo- 5α- lanost- 8-en-24-oate （meth