解磷菌株B25的筛选鉴定及其解磷能力-应用生态学报.PDF

应 用 生 态 学 报摇 2012年1月摇 第23卷摇 第1期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 ChineseJournal of Applied Ecology,Jan. 2012,23(1):235-239 解解磷菌株B25 的筛选、鉴定及其解磷能力* 贺梦醒摇 高摇 毅摇 胡正雪摇 徐摇 瑶摇 龙摇 瑞摇 孙庆业** (安徽大学资源与环境工程学院,合肥230601) 摘摇 要摇 从安徽省铜陵市铜官山尾矿库木贼根际分离筛选出多株解磷细菌,经过多次筛选纯 化获得一株解磷能力较好的菌株B25.采用透射电镜观察和DNA分子技术,确定此菌株属于 芽孢杆菌属.研究了解磷菌株B25 在培养168 h 内的解磷能力、溶液pH值以及菌株生长量的 变化情况,并比较了B25 在不同条件下的解磷能力.结果表明:解磷菌株B25 的解磷能力与溶 液pH值之间存在微弱的相关性,在碳源为葡萄糖、初始pH值为7.0、培养温度为30 益时解 磷效果较好. 关键词摇 解磷菌摇 解磷能力摇 16S rRNA 文章编号摇 1001-9332(2012)01-0235-05摇 中图分类号摇 Q939.96摇 文献标识码摇 A Screening, identification, and phosphate鄄solubilizing capability of phosphate鄄solubilizing bacterial strain B25. HE Meng鄄xing,GAOYi,HUZheng鄄xue,XUYao,LONG Rui,SUN Qing鄄 ye (School of Resources and Environmental Engineering,Anhui University,Hefei 230601, Chi鄄 na).鄄Chin.J.Appl.Ecol. ,2012,23(1):235-239. Abstract:Various phosphate鄄solubilizing bacterial strainswere isolatedfrom theHippochaete ramo鄄 sissimum rhizosphere in Tongguanshan copper tailings in Tongling of Anhui Province,East China. After manytimesof screeningandpurification,astrainB25with strongerphosphate鄄solubilizingca鄄 pability was obtained,which belonged toBacillus genus,as identified by transmission electron mi鄄 croscope and DNA molecular approaches. A culture experiment was conducted to study the phos鄄 phate鄄solubilizing capability of the B25 within 168 h and the variations of the medium pH and B25 growth aswell as the phosphate鄄solubilizing capability of B25 under different culture conditions. A weak correlation wasobservedbetweenthephosphate鄄solubilizing capability of B25 andthemedium pH. The B25 displayed a better phosphate鄄solubilizing capability when the carbon source was