Novel insight into the non-coding repertoire through deep sequencing analysis精选.pdfVIP

Nucleic Acids Research Advance Access published March 9, 2012 Nucleic Acids Research, 2012, 1–6 doi:10.1093/nar/gks228 Novel insight into the non-coding repertoire through deep sequencing analysis Ofer Isakov, Roy Ronen, Judit Kovarsky, Aviram Gabay, Ido Gan, Shira Modai and Noam Shomron* Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel Received October 12, 2011; Revised and Accepted February 23, 2012 ABSTRACT NcRNA expression profile can serve as an initial step for multiple sequence alignment-based phylogeny, Non-coding RNAs (ncRNA) account for a large homology and conservation studies (8). It can also be portion of the transcribed genomic output. This used for detecting RNA library preparation biases diverse family of untranslated RNA molecules play such as failure in tRNA and rRNA filtration, or undesir- a crucial role in cellular function. The use of ‘deep able abundance of transcript degradation products. sequencing’ technology (also known as ‘next gener- Differential expression of several types of ncRNAs can ation sequencing’) to infer transcript expression be of great value in a number of scientific fields such as: levels in general, and ncRNA specifically, is assessment of viral infection (9), diagnosis and prognosis becoming increasingly common in molecular and