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STATE OF MICHIGAN
DEPARTMENT OF ENVIRONMENTAL QUALITY
LANSING
RICK SNYDER DAN WYANT
GOVERNOR DIRECTOR
October 22, 2013
To All Interested Parties:
SUBJECT: State of Michigan Requirements for Whole Effluent Toxicity (WET) Test Methods
A) WET Test Methods
The State of Michigan requires the use of the following methods for all WET tests, except when
an approved plan requires different methods, or when tests are not being conducted for
compliance assessment (e.g., toxicity reduction evaluation tests). Of course, labs may use their
own protocols, provided that the protocols meet the requirements of the methods:
Chronic toxicity tests: EPA/600/4-91/002
Acute toxicity tests: EPA/600/4-90/027F
B) Specific WET Test Method and Reporting Requirements
Listed below is a compiled list of specific requirements and guidance for WET test methods and
reporting (see below). Failure to observe these requirements may invalidate test data.
1. Consulting laboratories shall initially validate to the Michigan Department of Environmental
Quality (MDEQ) their ability to perform each test method to be used prior to MDEQ
acceptance of WET data. A submittal of the results of five consecutive acceptable
reference toxicant tests and a comprehensive Standard Operating Procedure (SOP) for
the method are sufficient for validation. Please also include raw data from at least one set
of tests.
Consulting laboratories that have already validated
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