个体化裸鼠荷人肺癌肿瘤模型的建立.docVIP

个体化裸鼠荷人肺癌肿瘤模型的建立 王晓东 孙大强△ 李 志 白 悦 摘要 目的：建立人肺癌个体化可传代组织块动物模型。方法：组织块移植组将直径2 mm的人肺癌新鲜标本癌组织块移植于BALB/C裸鼠背部皮下组织内，每例人肺癌新鲜标本均移植入5只裸鼠体内，成瘤成功者为第一代。取第一代移植瘤组织块以相同方法移植于BALB/C裸鼠背部皮下组织内，成功者为第二代。重复上述传代方法得到第三代移植瘤。将人肺癌细胞株A549、H1795分别移植于BACL/C裸鼠背部皮下组织内，成瘤达500 mm3后取癌组织块。分别取实验组的原代人肺癌肿瘤及第一、二、三代移植瘤部分组织，及作为对照的细胞系移植瘤组织，固定于10%福尔马林溶液中，石蜡包埋、切片、HE 染色，光镜下观察比较其形态异同。结果：组织块移植组瘤细胞不仅保留了人肺癌癌细胞的异型性，还保持原有排列结构；而作为对照的细胞系移植瘤细胞失去了原有的排列结构，仅保留了癌细胞的异型性。结论：利用不同患者的手术切除肺癌标本所建立的裸鼠荷人肺癌肿瘤模型具有个体化特征，能够为肺癌个体化治疗的研究提供有效工具。 关键词 癌, 非小肺移植, , 肿瘤模型, 物小鼠, 近交BALBC 人The Establishment of Indivudal Human Lung Cancer Model by Xenotransplant Tumor Mass in Nude Mouse WANG Xiaodong ,SUN DA-qiang,Li Zhi,Bai Yue Tianjin Medical University Graduate School,Tianjin ,China Abstract Objective:To establish clinically relevant individuation nude mouse models of human non-small cell lung cancer,which can be stably passaged,so as to lay a foundation for the future individualized therapy study. Methods: Tumor implanting group: BALB/C nude mouse subcutaneously injected in the back with diameter 2mm tumor derived from human operative specimens were killed when the tumor mass grown to 800mm3.This is the first generation.Then,to establish the second generation, BALB/C nude mouse were injected in the back with diameter 2mm tumor derived from first generation tumor mass.the same method redone to gain the third generation tumor. Cell lines implanting group: BALB/C nude mouse subcutaneously injected in the back with 1×106 human lung cancer cell lines A549 or H1795 were killed when the tumor mass grown to 500mm3.The tissue of primary human lung cancer 、first 、second 、third generation implanting tumor and the group tumor were formalin-fixed、cut sections、HE stained,and then compared with the morphology by light?microscope.Results: The tumor cells of the umor implanting group not only retains the atypia of cancer cells, but also keep original arrangement structure; The cell lines implanting group tumor cells lose their arrangement structure, keep