Second Harmonic Imaging of Collagen英文资料.pdfVIP

2 Second-Harmonic Imaging of Collagen Guy Cox and Eleanor Kable Summary Molecules that have no center of symmetry are able to convert light to its second harmonic, at twice the frequency and half the wavelength. This only happens with any efficiency at very high light intensities such as are given by a pulsed laser, and because the efficiency of the process depends on the square of the intensity, it will be focal plane selective in exactly the same way as two-photon excitation of fluorescence. Because of its unusual molecular struct