斑马鱼血栓模型在中药抗血栓活性筛选中的适用性研究.pdf

·58· Chinese Journal of Information on TCM Jul.2017 Vol.24 No.7 斑马鱼血栓模型在中药抗血栓活性筛选中的适用性研究 樊娇娇，乔艺涵，赵崇军，倪媛媛，杨冉，冯娅茹，马志强，林瑞超 北京中医药大学中药学院中药品质评价北京市重点实验室，北京 100102 摘要：目的 考察斑马鱼血栓模型在中药抗血栓活性筛选中的适用性。方法 采用盐酸肾上腺素诱导建立 活体斑马鱼血栓模型。将斑马鱼随机分为空白组、模型组、阳性对照组、给药组，各组给予相应药物或胚胎培 养水。16 h 后，用邻联茴香胺染色液染色，计算斑马鱼心脏红细胞染色强度，进行定量分析；观察转基因斑马 鱼血小板聚集情况，进行定性分析。结果 与模型组比较，100 μg/mL 丹酚酸 B ，300、900 μg/mL 丹参水提取 物，45 μg/mL 95% 乙醇提取物，400 、1200 μg/mL 消栓通络片均能明显抑制斑马鱼的血栓形成（P ＜0.01 ）。 结论 斑马鱼血栓模型对中药抗血栓活性筛选具有良好的适用性。 关键词：斑马鱼血栓模型；中药；活性筛选；适用性 DOI：10.3969/j.issn.1005-5304.2017.07.014 中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2017)07-0058-04 Applicability of Zebra Fish Thrombosis Model in Antithrombotic Activity Screening of Chinese Materia Medica FAN Jiao-jiao, QIAO Yi-han, ZHAO Chong-jun, NI Yuan-yuan, YANG Ran, FENG Ya-ru, MA Zhi-qiang, LIN Rui-chao (School of Chinese Materia Medica, Beijing Key Laboratory of Traditional Chinese Medicine Quality Evaluation, Beijing University of Chinese Medicine, Beijing 100102, China) Abstract: Objective To investigate the applicability of zebra fish thrombosis model in antithrombotic activity screening of Chinese materia medica. Methods The living zebra fish thrombosis model was induced by adrenaline hydrochloride. Zebra fish were randomly divided into blank control group, model group, positive medicine group and medication group. Each group was given the corresponding medicine or embryo culture water. O-anisidine staining solution was used to stain and calculate the staining intensity of erythrocytes in zebra fish heart, and quantitative analysis was carried out. The platelet aggregation of transgenic zebra fish was observed and under qualitative analysis. Results Compared with the model group, 100 μg/mL salvianolic acid B, 300,