柿EST-SSR引物的开发及筛选 Development and screening of EST-SSR markers in Diospyros kaki Thunb.pdfVIP
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柿EST-SSR引物的开发及筛选 Development and screening of EST-SSR markers in Diospyros kaki Thunb
第30卷第3期 经济林研究 V.01.30No.3
2012年9月 NonwoodForestResearch Sep.2012
柿EST-SSR引物的开发及筛选
吴硕1,傅建敏1,乌云塔娜2,梁玉琴1,李芳东1
(1.中国林业科学研究院经济林研究开发中心,河南郑州450003:
2.中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004)
474条柿属EST序列开发柿EST-SSR引物,并用于
摘 要:为丰富柿树SSR引物来源,利用从NCBI下载的9
分析7个柿品种的遗传多样性。EST序列经去除低质量及冗余片段后拼接成1390条重叠序列和4097条无重复
引物的31%,其中14对引物有多态性,占可扩增引物的45.2%。同时发现引物68是临潼板柿的特异引物。引
的特异性标记位点。
关键字:柿;EST;SSR标记
中图分类号:$665.2 文献标志码:A
and ofEST-SSRmarkersin
Developmentscreening
WE YUN
Shu01,FUJian—minl,WUTa-na2,LIANG
Yu—qinl,LIFang—don91
(1.Non-timberForestResearchandDevelopmentCenter,ChineseAcademy
2.The LabofNon-woodForestProduct South
Key ofForestryMinistry,CentralUniversity
410004,Hunan,China)
Changsha
ordertoenrichSSR sourcesof kaki makerswere from9474
Abstract:In Thunb.,SSR
primer Diospyros developed
L.in and wereusedto diversities7 cultivarofD.kaki.1390
ESTsof NCBIdatabase
Diospyros they analyze among
and4097 wereassembled andredundant
repeatedsequences non--repeatedsequences byremovinglow·-quality fragments
1
inESTs SSRsin601 EST weresearched for0.96%of
total,540
sequences.In sequences out,accounting ESTs,among
which61.06%ofSSRswasdinucleotides.Onehundred were forPCR of7cultivars.
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