peg蛋白的超滤：污染和浓差极化效应.pdfVIP

Journal of Membrane Science 319 (2008) 206–213 Contents lists available at ScienceDirect Journal of Membrane Science j o u rn al h omepage: www.elsevi er.c om /locate/memsci Ultrafiltration of PEGylated proteins: Fouling and concentration polarization effects Boksoon Kwon, Jessica Molek, Andrew L. Zydney ∗ Department of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USA a r t i c l e i n f o a b s t r a c t Article history: One of the critical factors governing the application of membrane technology for the commercial produc- Received 8 October 2007 tion of PEGylated proteins is membrane fouling. The objective of this study was to examine the effects Received in revised form 27 February 2008 of PEGylation on the filtrate flux and fouling characteristics during ultrafiltration of a model PEGylated Accepted 23 March 2008 protein using both fully and partially retentive regenerated cellulose and polyethersulfone membranes. Available online 30 March 2008 The physical characteristics of the native and PEGylated proteins were evaluated by size exclusion chro- matography, dynamic light scattering, capillary electrophoresis, and reverse-phase chromatography. The Keywords: filtration behavior with the cellulose membranes was dominated by concentration polarization effects, Membrane fouling PEGylation with the lower flux for the PEGylated protei