葡萄藻中碳氢化合物提取与gc-ms分析条件比较研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.5 葡萄藻中烷烃化合物的GC-MS分析条件比较 魏东，熊爽 （华南理工大学轻工与食品学院，广东广州 510640） 摘要：通过单因素分析，对不同溶剂、温度和时间条件下提取葡萄藻微量藻粉中碳氢化合物的效果进行了比较，获得了最佳提 取条件为：40 mg 藻粉悬浮于正己烷中，超声处理（35 ℃，70 Hz ）半小时后再于35 ℃下提取 10 h，碳氢化合物得率最高，占藻粉干 重的 2.95‰。比较获得了 GC-MS 分析的程序升温优化条件，发现处于对数期的葡萄藻微量冻干藻粉中含量最高的碳氢化合物都是 C22-C31 长链烷烃，其组成与含量分别为：二十二烷（50.40 μg/g ）、二十四烷（209.17 μg/g ）、二十五烷（351.45 μg/g ）、9-辛基十 七烷（472.38 μg/g ）、二十七烷（540.48 μg/g ）、二十八烷（542.24 μg/g ）、角鲨烯（473.60 μg/g ）、正三十一烷（306.86 μg/g ）。 该提取和分析过程具有样品量少、时间短、得率高和操作简单的优点，可作为葡萄藻碳氢化合物分析检测的一种快速高效途径。 关键词：葡萄藻；碳氢化合物；提取 文章篇号：1673-9078(2014)5-280-286 Comparative Study of Extraction Conditions and GC-MS Analysis of Hydrocarbons from Botryococcus sudeticus WEI Dong, XIONG Shuang (School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China) Abstract: Based on ANOVA analysis of the solvents, temperatures and time, the optimal conditions for hydrocarbons extraction from small amount of algal powder of Botryococcus sudeticus were obtained as follows: 40 mg algal powder suspended in n-hexane was ultrasonicated at 35 ℃ and 70 Hz for 30 min, then extracted at 35 ℃ for 10 hours. The highest yield of hydrocarbons accounted for 2.95 ‰ of dry weight was obtained under above conditions. The programmed procedure for GC-MS analysis was compared and optimized, and long-chain alkanes from C22 to C31 were found as the dominant hydrocarbons in the lyophilized powder of Botryococcus sudeticus from the cells in exponential phase. They were consisted of docosane, tetracosane, pentacosane, 9-octylheptadecane, heptacosane, octacosane, squalene and hentriacontane, with contents of 50.40, 209