水稻白叶枯菌OS198中talR265基因的克隆及功能分析.doc

水稻白叶枯菌OS１９８中talR２６．５基因的克隆及功能分析 朱引引　刘永庭　李士河　宋从凤 (南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,南京２１００９５;通讯联系人,EＧmail:cfsong＠njau． edu．cn) CloningandFunctionalCharacterizationoftalR２６．５GenefromXanthomonas oryzaepv．oryzaeOS１９８Strain ZHUYinＧyin,LIUYongＧting,LIShiＧhe,SONGCongＧfeng (CollegeofPlantProtection/KeyLaboratoryofIntegratedManagementofCropDiseasesandPests,MinistryofEducation, NanjingAgriculturalUniversity,Nanjing２１００９５,China;Correspondingauthor,EＧmail:cfsong＠njau．edu．cn) ZHUYinyin,LIUYongting,LIShihe,etal．CloningandfunctionalcharacterizationoftalR２６．５genefrom Xanthomonasoryzaepv．oryzaeOS１９８strain．ChinJRiceSci,２０１４,２８(４):３４３Ｇ３５０． Abstract:AstherepresentativestrainofXanthomonasoryzaepv．oryzae(Xoo)Chinarace６,OS１９８displayedlow virulencein２６ricevarieties．talR２６．５isatranscriptionactivatorＧlike(tal)geneclonedfromthegenomicDNAof OS１９８．TALR２６．５had２６．５tandemＧrepeatsof３４aminoacidresiduesandsharedover９９％homologywiththeTAL effectorsAvrXa７Ｇ３MofPhilippinesstrainPXO３５７andYP_４５１８９５ofJapanesestrainMAFF３１１０１８．Therepeat domainswereidenticalforthethreeproteins．TheintroductionoftalR２６．５intoPXO９９Adecreasedthevirulenceand suppressedthebacterialpopulationsinthreenearＧisogenicricelinesIRBB８,IRBB１４andIRBB２１４comparedwiththe wildtypePXO９９A．ThetransformantstrainsshowednodifferenceofvirulencewithPXO９９Ain２２otherricevarieties includingIRBB７．TheinducedresistanceofIRBB８,IRBB１４andIRBB２１４againsttheinfectionofPXO９９Aharboring talR２６．５wasimplementedbythesuppressionofdiseasesusceptibilitygeneOs８N３．PXO９９A/talR２６．５aroused enhancedhypersensitiveresponseintobaccoleavescomparedwithPXO９９A．TheresultsindicatedthatthetalR２６．５ geneofOS１９８mighthavedualfunctionsofvirulenceandavirulence． Keywords:Xanthomonasoryzaepv．oryzae;transcriptionactivatorＧlikegene;virulence;hypersensitiveresponse 朱引引,刘永庭,李士河,等．水稻白叶枯菌OS１９８中talR２６．５基因的克隆及功能分析．中国水稻科学,２０１４,２８ (４):３４３Ｇ３５０． 摘　要:类似转录激活因子(transcriptionactivatorＧlike,tal)基因是水稻白叶枯菌中一类重要的致病相关基因,其编码蛋白 属于AvrBs３/pth家族.tal基因的挖掘和功能分析是揭示白叶枯病菌致病遗传机制的基础.OS１９８是水稻白叶枯病菌中国 菌株６号小种的代表菌株,在供测试的２６个水稻品种上毒力较弱.从OS１９８的基