大豆转录因子GmWRI1基因的分离及其转录激活特性分析.pdfVIP

中国油料作物学报 2017，39(4)：447—453 Chinese Oil Journalof Sciences Crop 大豆转录因子GmWRll基因的分离 及其转录激活特性分析 陈欢，邓文波5，张莹，刘伟灿，于潇潇，王法微，王野，庄帅兵，张永红，王真慧+，李海燕4 (吉林农业大学生命科学院，吉林长春，130118) 摘要：为探讨大豆转录因子GmWRll在大豆种子油脂形成过程中的调控作用，本研究从大豆吉育72花后35d 的种子中克隆得到GmWRll全长cDNA序列，并对该基因编码的蛋白的理化性质、结构功能进行了初步分析；通过 AHl09酵母菌株中，结果表明GmWRll具有转录激活活性，属于转录激活子。 关键词：大豆；转录因子；GmWRll；油脂含量 中图分类号：$565．103文献标识码：A 文章编号：1007—9084(2017)04—0447—07 Isolationand activationofGmWRll factorfrom transcriptionalanalysis transcription soybean CHEN Wen—bo Huan，DENG 9，ZHANGYing，LIUWei—can，YUXiao—xiao，WANGFa—wei， WANG Zhen—hui Ye，ZHUANG Shuai—bing，ZHANGYong—hong，WANG4，LIHai—yan+ (CollegeofLifeScience，Jilin 130118，China) AgriculturalUniversity，Changchun theroleofGmWRll factorsin oil in Abstract：To explore transcriptionregulatingdevelopmentsoybean，Gm— WRll wasclonedfromseedsof 72atthe35th after itsdeduced gene Jiyu day flowering，and structure were andfunction characterizethe ofGmWRllatdifferentsta- preliminaryanalyzed．To expressionpattern after of 72werealsodetected PCR．Theresults ges Jiyu real—time showedthatthe flowering，seeds by quantitative levelofGmWRllcorrelatewiththe seedsoil as expression may soybean vectorwasconstructedrecombinantDNA and in its expression using technologyexpressedyeast(AHl09)toanalyze activation resul