A reductionist cell free major histocompatibility complex class II antigen processing system identifies immunodominant epitopes英文文献.pdfVIP

t e c h n i c a l r e p o r t s A reductionist cell-free major histocompatibility complex class II antigen processing system identifies immunodominant epitopes 1,7,8 1,8 2 3 3 4 Isamu Z Hartman , AeRyon Kim , Robert J Cotter , Kimberly Walter , Sarat K Dalai , Tatiana Boronina , 2 5 5 5 4,6 1,3 Wendell Griffith , David E Lanar , Robert Schwenk , Urszula Krzych , Robert N Cole Scheherazade Sadegh-Nasseri . d e v Immunodominance is defined as restricted responsiveness developed to identify MHC-restricted peptides that may elicit strong r e of T cells to a few selected epitopes from complex antigens. T cell responses. One of these approaches uses large arrays of overlap- s e + r Strategies currently used for elucidating CD4 T cell epitopes ping synthetic peptides that map the entire amino acid sequence of s are inadequate. To understand the mechanism of epitope an antigenic protein to identify MHC-restricted peptides and, con- t h g selection for helper T cells, we established a cell-free sequently, putative immunodominant epitopes. The large number i r l antigen processing system composed of defined proteins: of protein sequences available for mapping is now making peptide- l A human leukocyte antigen-DR1 (HLA-DR1), HLA-DM and mapping experiments highly expensive, labor intensive and often . cathepsi