A granulocyte macrophage colony–stimulating factor and interleukin 15 fusokine induces a regulatory B cell population with immune suppressive properties英文文献.pdfVIP

a r t i c l e s A granulocyte-macrophage colony–stimulating factor and interleukin-15 fusokine induces a regulatory B cell population with immune suppressive properties 1 1 2,3 1 1 1 Moutih Rafei , Jeremy Hsieh , Simone Zehntner , MengYang Li , Kathy Forner , Elena Birman , 1 1 4 1,5 Marie-Noëlle Boivin , Yoon Kow Young , Claude Perreault Jacques Galipeau . d We have previously shown that a granulocyte-macrophage colony–stimulating factor (GM-CSF) and interleukin-15 (IL-15) e v ‘fusokine’ (GIFT15) exerts immune suppression via aberrant signaling through the IL-15 receptor on lymphomyeloid cells. r e We show here that ex vivo GIFT15 treatment of mouse splenocytes generates suppressive regulatory cells of B cell ontogeny s e + r (hereafter called GIFT15 B cells). Arising from CD19 B cells, GIFT15 B cells express major histocompatibility complex s reg reg t class I (MHCI) and MHCII, surface IgM and IgD, and secrete IL-10, akin to previously described B10 and T2-MZP B cells, h reg g but lose expression of the transcription factor PAX5, coupled to upregulation of CD138 and reciprocal suppression of CD19. i r l Mice with experimental autoimmune encephalomyelitis went into complete remission after intravenous infusion of GIFT15 l A B cells p