姜黄素分光光度法测定硼分析方法研究-化学毕业论文.docxVIP

毕业论文题目：姜黄素分光光度法测定硼的分析方法研究（一）姓名：孙国涛学号： 2008114168 院系：理学院化学系专业：化学专业年级： 2008级指导教师：孙柏林赵海香二〇一二年六月目录1引言22原理33仪器及试剂33.1仪器33.2试剂33.3溶液的配置34分光光度法测定44.1显色反应44.2可见光度法测定45结果与讨论45.1显色条件的优化45.1.1显色剂用量的确立45.1.2显色时间的确立55.1.3显色水浴温度的优化55.1.4显色后测定时间的影响65.1.5显色剂的稳定性试验（常温下）65.2影响因素研究75.2.1酸的影响75.2.2盐的影响85.2.3不同介质85.3实验结果96结论107资金支持：11参考文献11致谢11姜黄素分光光度法测定硼的分析方法研究（一）孙国涛河北北方学院理学院化学系，河北张家口075000摘要：本文确立了在分光光度法测量硼含量的最佳测定条件，探讨了不同因素对实验结果的影响，为姜黄素分光光度法测定硼含量提供参考。实验采用姜黄素为显色剂，以乙醇为介质，在水浴蒸干乙醇溶解后测量其吸光度，确立最佳显色条件，考察不同因素（包括酸、盐和溶剂）对该方法的影响。实验得出的优化条件为：显色剂用量4mL，最佳显色时间为15min，最佳水浴温度55 ±3oC，显色测定时间在4天以内。方法的相对标准偏差为0.2%，标准曲线的相关线性系数为0.9994。关键词：姜黄素，硼, 分光光度法The study of analysis method for determination of boron with curcumin spectrophotometry (one)Sun GuotaoDepartment of chemistry, College of Science,Hebei North University, Zhangjiakou Hebei 075000Abstract: This paper established the optimum conditions of determinating boron with curcumin spectrophotometry, discussed the effect of different factors on the results, and provided a reference for the curcumin spectrophotometric determination of boron content. For experiments, the curcumin was as chromogenic reagent, thealcohol was as the medium, the absorbance was determated in the water bath evaporated alcohol.The experiments optimized the chromogenic conditions and studied the influence of different factors such as acide, salt and solvent on the method. The optimun experimental conditions are: the curcumin dosages of 4mL, the chromogenic time of 15min, the water bath temperature of 55 ± 3 o C and determination time within four days. Based as experiment method, the RSD was of 0.2%, the linear coefficient of standard curve was 0.9994. Key words: Curcumin, boron, spectrophotometry1引言硼（B），原子序数5，自然界中硼只以其化合物形式存在，如硼酸（H3BO3）、硼砂（Na2B4O7.10H2O）等，在动物和植物中存在着结合态的痕量硼。人类、动物和植物都需要硼，即硼是人体必需的微量元素，但是对人类而言，硼酸、硼砂、硼酸钠都是低毒类蓄积性毒物，每天口服100mg，可引起慢性中毒[1]，容易造成畸形。医学研究提倡人类通过食用含硼较高的豆类、多叶的蔬菜、水果补充硼，即植物中原生态的硼对人类和动物是安全的和有益的。但是由于硼砂具有防腐、增加食品的口感、感官度、韧性感和嚼劲度的作用，在我国的传统小吃中