多种药材的增荧光薄层鉴别研究-中国现代应用药学.pdf

·药物分析与检验· 多种药材的增荧光薄层鉴别研究 李晓燕1 2 1 1 2 ，韩桂茹 ，许红辉 ，李向军 ，封淑华 (1.河北以岭医药研究院，石家庄 050035 ；2.河北省药品检验所，石家庄 050011) 摘要：目的 拓宽薄层鉴别检测途径，提高中药成方制剂中的薄层鉴别增订率。方法 将各药材的样品溶液点于薄层板 上，展开后，进行不同方式的增荧光反应研究。结果 药材中多种无检测信息的成分呈现出不同颜色的荧光，斑点清晰， 检测灵敏度提高，信息量增大。结论 本研究有效拓宽了薄层鉴别检测途径，使多种无检测信息的中药材，能够被高灵 敏度、准确地进行定性和定量控制。 关键词：薄层鉴别；增荧光反应；荧光斑点；中药材 中图分类号：R931.5 文献标志码：B 文章编号：1007-7693(2011)02-0153-04 Study on the TLC Identification of Several Herbal Drugs by Increasing Fluorescence Reaction 1 2 1 1 2 LI Xiaoyan , HAN Guiru , XU Honghui , LI Xiangjun , FENG Shuhua (1.Hebei Yiling Medicine Institute, Shijiazhuang 050031, China; 2.Hebei Institute for Drug Control, Shijiazhuang 050011, China ) ABSTRACT ：OBJECTIVE To broaden TLC identification detection way, improve the detection ratio of herbal drugs in Chinese traditional patent preparations. METHODS Apply the sample solution in thin plate, after developing, study increasing fluorescence reaction by different ways. RESULTS The various components that without detection information in herbal drugs showed different color fluorescence and clear spots, which improved detection sensitivity and provided more detection information. CONCLUSION The study effectively broadened TLC identification application fields, made several herbal drugs that without detection information be able to be qualitative and quantitative controlled with high sensitivity and accuraey. KEY WORDS: TLC identification; increasing fluorescence reaction; fluorescence spots; Chinese herbal drugs 目前在国家各类中药制剂质量标准中，其药 蟾酥对照药材(批号：121132-20001)、白术对 材的薄层鉴别增订率低，平均约为处方中药材总 照药材(批号：120925-200708)、连翘对照药材(批号： 量的 30%，无法全方位监控制剂质量。尤其是提 120908-200310)、人工牛黄对照药材(批号：121197- 取制剂，药材的显微特征已不复存在，显微鉴别 200502) 、乌药对照药材(批号：1096-200001