plk1和rb在皮肤鳞状细胞癌中的表达及意义word论文.docxVIP

英汉缩略语名词对照英文缩写英文全称中文名称APCAnaphase-promotingcomplex后期促进复合物APC/CAnaphase-promotingcomplex/cyclosome后期促进复合物/细胞周期体CSCCCutaneoussquamous cellcarcinoma皮肤鳞状细胞癌CdklCellcycledependentkinase1细胞周期素依赖性激酶1DABDiaminobenzidine二氨基联苯胺Emi1Earlymitoticinhibitor1早期有丝分裂抑制剂1HBO1Histoneacetyltransferase bindingtotheoriginrecognition complex1组蛋白乙酰转移酶绑定源识别复合物1HDAC1Histonedeacetylase1组蛋白脱乙酰酶1LDHLactate dehydrogenase乳酸盐脱氢酶Mklp-1Mitotic kinesin-likeprotein有丝分裂驱动蛋白样蛋白1NlpNinein-likeprotein中心体蛋白NLSNuclearlocalization signal核定位信号NudCNucleardistributionproteinC核迁移蛋白CPlk1Polo-likekinase1保罗样激酶1pre-RCPrereplicative complex复制前复合物PBDPoloBoxDomain保罗盒结构域PBSRbPhosphatebuffersalineRetinoblastoma磷酸盐缓冲液成视网膜细胞瘤RhoGEFTCTPRhoguaninenucleotideexchangefactorTubulin-stabilizingproteinRho鸟嘌呤核苷酸交换因子微管稳定蛋白Plk1和Rb在皮肤鳞状细胞癌中的表达及意义硕士研究生：曾芬导师：李慎秋教授中文摘要目的：检测Plk1及Rb在皮肤鳞状细胞癌(CSCC)及正常皮肤组织中的表达情况，探讨两者在CSCC发生发展中作用，以期为CSCC早期筛查提供重要线索，同时寻求治疗的新靶点。方法：收集2011-2013年保存的CSCC组织蜡块共20份，病理诊断明确，另选取20例皮肤科手术取下的正常皮肤为正常对照，采用免疫组化Elivision法检测20例CSCC和20例正常皮肤组织中Plk1和Rb的表达情况。结果：Plk1蛋白表达于鳞癌细胞和正常皮肤细胞的胞浆和胞核中，而Rb主要表达于胞核中。在CSCC中Plk1蛋白的阳性表达显著高于正常对照组，差异有高度统计学意义（P0．01)，Rb蛋白的阳性表达显著低于正常对照组，差异有统计学意义（P0．05)。结论：与正常皮肤相比，CSCC中Plk1蛋白的表达增高，而Rb蛋白的表达减少，说明Plk1和Rb参与了CSCC的发生发展过程。关键词：皮肤鳞状细胞癌Plk1RbExpressionandSignificanceofPlk1andRb in Cutaneous SqumamousCellCarcinomaPostgraduate:ZengFen Tutor:ProfessorLishenqiuAbstractObjective：Toexaminetheexpressionofpolo-like kinase1andretinoblastomaproteinin cutaneoussquamouscellcarcinomaandnormalskintissues,investigatetheroleofPlk1 andRbintheCSCCandtrytoprovidecluestodetectCSCC,aswellaslookfornewtheropiesforthedisease.Methods：Theparaffinfixedspecimenof20CSCCcaseswerecollectedinoutpatientdepartmentfrom2011to2013,whichwerepathologicallydiagnosed.Thecontrolswereobtainedfromsurgicalremovalofnormalskinof20patientsindermatologyoutpatientdepartment.WeelucidatetheexpressionofPlk1andRbinCSCCandnormalskintissues byimmunohistochemistryElivisionmethod.Results：TheexpressionofPlk1waslocalizedinthecytoplasmandnucleioftumorcellsandnormalcells,whilethee