幽门螺杆菌毒性噬菌体分离纯化及模拟治疗研究.PDFVIP

·304 · 南方医科大学学报（JSouthMedUniv） 2011;31(2) ·基础研究· 幽门螺杆菌毒性噬菌体分离纯化及模拟治疗研究 万学勤，唐冬生，刘爱平，谭淑仪，李万可兰，邝 佳，李宏鸣 佛山科学技术学院医学院，广东 佛山 528000 摘要：目的分离纯化幽门螺杆菌（Hp ）的野生型毒性噬菌体，并采用体外模拟试验，探讨借口腔Hp 保护噬菌体突破胃液屏 障、释放子代噬菌体、侵染并治疗胃肠Hp 感染的方法。方法以烛缸法培养Hp ，用单层平板和双层平板实验分离纯化噬菌 体，采用体外模拟胃液环境处理对照组噬菌体和Hp 保护的实验组噬菌体，以双层平板实验检测处理后噬菌体的杀菌效 果。结果经过模拟胃液处理后，噬菌体溶菌活性随处理时间延长而降低，处理1.5h 的实验组依然可形成噬斑，对照组不 形成噬斑。结论口腔Hp 可以帮助噬菌体抵抗胃液。 关键词：幽门螺杆菌；毒性噬菌体；生物治疗 中图分类号：Q939.4892 文献标志码：A 文章编号：1673-4254（2011）02-0304-04 Isolation of a wild-type virulent phage of Helicobacter pylori and its simulated treatments of gastrointestinal Hp in vitro WAN Xue-qin, TANG Dong-sheng, LIU Ai-ping, TAN Shu-yi, LI Wan-kelan, KUANG Jia, LI Hong-ming Medical College of Foshan University, Foshan 528000, China Abstract: Objective To isolate the wild-type virulent phage of Helicobacter pylori (Hp) and simulate the treatments in vitro to investigate the methods for oral Hp-assited penetration of the phage through the gastric barrier and offspring phage release for infection and treatment of gastrointestinal Hp. Methods The Hp strain was cultured with the candle cylinder method and the virulent phage was isolated by single plate or double plate experiment. A simulated gastric juice was applied and the bactericidal effect of the phage was tested with double flats experiment. Results After a 1.5-h treatment in simulated gastric juice, the orally derived Hp-borne phage was still capable of forming plaques while the control phage was not. Conclusion The oral Hp can help the phage resist the gastric juice and then infect the gastrointestinal Hp. Key words: Helicobacter pylori; virulent phage; biotherapy 幽门螺杆菌（Helicobacter pylori; Hp ）被世界卫